Faculty Information - HARAGUCHI Yuuji

Books

Published papers

Academic conference presentation

Research areas

Academic society memberships

Education

（Last updated : 2025-12-01 11:19:39）

HARAGUCHI Yuuji
Department Graduate School of Medical Science, Graduate School of Medical Science

Position Associate Professor (Fixed Term)

■ Books

1.	Chapter contribution	Tissue Fabrication and Regeneration by Cell Sheet Technology, Handbook of Intelligent Scaffolds for Tissue Engineering and Regenerative Medicine 2017/05
2.	Chapter contribution	Cell Scooper: A Device for the Rapid Transfer of Living Cell Sheet, Hyper Bio Assembler for 3D Cellular Systems 2015/07
3.	Chapter contribution	Cell Sheet-Based Myocardial Patches, Myocardial Tissue Engineering 2015/01
4.	Chapter contribution	Cell sheet-based myocardial tissue engineering, Myocardial Tissue Engineering 2015/01
5.	Chapter contribution	Cell Sheet-Based Tissue Engineering, Myocardial Tissue Engineering 2015/01
6.	Chapter contribution	Future Developments, Myocardial Tissue Engineering 2015/01
7.	Chapter contribution	Introduction, Myocardial Tissue Engineering 2015/01
8.	Chapter contribution	Scaffold-based myocardial tissue engineering, Myocardial tissue engineering 2015/01
9.	Chapter contribution	Cell sheet technology for cardiac tissue engineering, Methods in Molecular Biology (Cardiac Tissue Engineering) 2014/06
10.	Chapter contribution	Cell-Based Therapy for Cardiovascular Injury, Emerging Trends in Cell and Gene Therapy 2013/07
11.	Chapter contribution	Cell sheet nano technology engineering and applications to cardiology, Nanomedicine and the Cardiovascular System 2011/10
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■ Published papers

1.	Original article	Microbial amino acid/protein production using nitrogen-fixing cyanobacteria without animal- or crop-derived nutrients 2025/06
2.	Original article	Nitrogenous fertilizer-free microalgal culture using nitrogen-fixing cyanobacteria-derived extracts for sustainable food production 2025/01
3.	Original article	Muscle cell proliferation using water-soluble extract from nitrogen-fixing cyanobacteria Anabaena sp. PCC 7120 for sustainable cultured meat production. 2023/10/04
4.	Original article	Proliferation of mammalian cells with Chlorococcum littorale algal compounds without serum support. 2023/10
5.	Original article	Circular cell culture for sustainable food production using recombinant lactate-assimilating cyanobacteria that supplies pyruvate and amino acids. 2023/06/16
6.	Original article	Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production 2023/01/10
7.	Original article	Production of scaffold-free cell-based meat using cell sheet technology. 2022/09
8.	Original article	Proliferation and differentiation of primary bovine myoblasts using Chlorella vulgaris extract for sustainable production of cultured meat. 2022/03/30
9.	Original article	Microalgal culture in animal cell waste medium for sustainable 'cultured food' production. 2021/11
10.	Original article	Cell detachment from monolayer- and bilayer-type gold nanoparticle-containing collagen coatings by visible laser irradiation for cell sorting applications 2021/07/13
11.	Original article	Three-dimensional tissue fabrication system by co-culture of microalgae and animal cells for production of thicker and healthy cultured food. 2021/03
12.	Original article	Mammalian cell cultivation using nutrients extracted from microalgae. 2020/03
13.	Original article	Analysis of force vector field during centrifugation for optimizing cell sheet adhesion. 2019/10
14.	Original article	Rapid creation system of morphologically and functionally communicative three-dimensional cell-dense tissue by centrifugation. 2018/12
15.	Original article	Contractile force measurement of human induced pluripotent stem cell-derived cardiac cell sheet-tissue 2018/05
16.	Review article	Fabrication of three-dimensional cardiac tissue from a 'symbiotic recycling culture' combining algae and mammalian cells 2018/03
17.	Original article	Rapid fabrication of detachable three-dimensional tissues by layering of cell sheets with heating centrifuge 2018/01
18.	Original article	Real-time quantitation of internal metabolic activity of three-dimensional engineered tissues using an oxygen microelectrode and optical coherence tomography. 2017/05
19.	Original article	Optical coherence microscopy of living cells and bioengineered tissue dynamics in high-resolution cross-section. 2017/04
20.	Original article	Three-dimensional human cardiac tissue engineered by centrifugation of stacked cell sheets and cross-sectional observation of its synchronous beatings by optical coherence tomography 2017/02
21.	Original article	Thicker three-dimensional tissue from a symbiotic recycling system combining mammalian cells and algae 2017/01
22.	Review article	Cross-sectional observation of three-dimensional cell sheet-tissues by OCT 2016/10
23.	Original article	In situ cross-linked electrospun fiber scaffold of collagen for fabricating cell-dense muscle tissue 2016/06
24.	Original article	Preparation of thermoresponsive nanostructured surfaces for tissue engineering 2016/03
25.	Original article	TRPV-1-mediated elimination of residual iPS cells in bioengineered cardiac cell sheet tissues. 2016/02
26.	Original article	Electrophysiological analysis of mammalian cells expressing hERG using automated 384-well-patch-clamp 2015/12
27.	Original article	Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation 2015/12
28.	Original article	Simple suspension culture system of human iPS cells maintaining their pluripotency for cardiac cell sheet engineering 2015/12
29.	Original article	Time Course of Cell Sheet Adhesion to Porcine Heart Tissue after Transplantation 2015/10
30.	Original article	Real-time, noninvasive optical coherence tomography of cross-sectional living cell-sheets in vitro and in vivo 2015/08
31.	Original article	Noninvasive cross-sectional observation of three-dimensional cell sheet-tissue-fabrication by optical coherence tomography 2015/06
32.	Original article	Oxygen consumption of human heart cells in monolayer culture 2014/09
33.	Review article	Latest status of the clinical and industrial applications of cell sheet engineering and regenerative medicine 2014/01
34.	Original article	Chondrocyte differentiation of human endometrial gland-derived MSCs in layered cell sheets 2013/11
35.	Original article	A device for the rapid transfer/transplantation of living cell sheets with the absence of cell damage 2013/08
36.	Review article	Cell sheet transplantation for heart tissue repair 2013/08
37.	Original article	New isolation system for collecting living cells from tissue 2013/01
38.	Original article	Creation of human cardiac cell sheets using pluripotent stem cells 2012/08
39.	Original article	Fabrication of functional three-dimensional tissues by stacking cell sheets in vitro 2012/04
40.	Review article	Cell Therapy and Tissue Engineering for Cardiovascular Disease 2012/02
41.	Review article	Scaffold-free Tissue Engineering Using Cell Sheet Technology 2012/02
42.	Original article	Creation of mouse embryonic stem cell-derived cardiac cell sheets. 2011/10
43.	Review article	Regenerative therapies using cell sheet-based tissue engineering for cardiac disease 2011/10
44.	Original article	Electrical interaction between cardiomyocyte sheets separated by noncardiomyocyte sheets in heterogeneous tissues 2010/06
45.	Original article	Design of prevascularized three-dimensional cell-dense tissues using a cell sheet stacking manipulation technology. 2010/05
46.	Original article	Development of a new assay system for evaluating the permeability of various substances through three-dimensional tissue 2009/10
47.	Review article	Cell sheet engineering for regenerative medicine: From the viewpoint of inflammation 2007/05
48.	Original article	Electrical coupling of cardiomyocyte sheets occurs rapidly via functional gap junction formation. 2006/09
49.	Review article	Tissue reconstruction using cell sheet engineering and its application to regenerative medicine 2004
50.	Original article	CD4-Dependent and CD4-independent utilization of coreceptors by human immunodeficiency viruses type 2 and simian immunodeficiency viruses 2000/12/05
51.	Original article	Infection of mesangial cells with HIV and SIV: identification of GPR1 as a coreceptor 2000/08
52.	Original article	Apelin peptides block the entry of human immunodeficiency virus (HIV) 2000/05/04
53.	Original article	Inhibition of cell-free human T-cell leukemia virus type 1 infection at a postbinding step by the synthetic peptide derived from an ectodomain of the gp21 transmembrane glycoprotein 1999/11
54.	Original article	Inhibition of HIV-1 infection by zinc group metal compounds 1999/09
55.	Original article	Heat shock cognate protein 70 is a cell fusion-enhancing factor but not an entry factor for human T-cell lymphotropic virus type I 1999/08/02
56.	Original article	Changes in and discrepancies between cell tropisms and coreceptor uses of human immunodeficiency virus type 1 induced by single point mutations at the V3 tip of the env protein 1999/07/05
57.	Original article	Isolation and characterization of a monoclonal antibody that inhibits HIV-1 infection 1999/07
58.	Original article	Human complement component C1q inhibits the infectivity of cell-free HTLV-I 1998/11
59.	Original article	Identification of membrane antigens important for adsorption of human T-cell leukaemia virus type I 1998/11
60.	Original article	Identification of the chemokine receptor TER1/CCR8 expressed in brain-derived cells and T cells as a new coreceptor for HIV-1 infection 1998/02
61.	Original article	Inhibition of plating of human T cell leukemia virus type I and syncytium-inducing types of human immunodeficiency virus type 1 by polycations 1997/11/20
62.	Original article	Different properties of human T-cell leukemia virus type I prepared from 8C cat cells and human cells 1997/04
63.	Original article	Synthetic studies on the relationship between anti-HIV activities and micelle forming abilities of various alkylated glycyrrhetinate diglycoside sodium sulfates and related compounds 1996/08
64.	Original article	Sulfated colominic acid: an antiviral agent that inhibits the human immunodeficiency virus type 1 in vitro 1996/06
65.	Original article	Inhibition of human immunodeficiency virus type 1 infectivity by a new amine bellenamine 1996/03
66.	Original article	New bellenamine homologs inhibiting human immunodeficiency virus type I infectivity 1996/01
67.	Original article	Detection of neutralizing antibodies against human T-cell leukemia virus type 1 using a cell-free infection system and polymerase chain reaction 1994/11/01
68.	Original article	Inhibition of adsorption of human T-cell-leukemia virus type 1 by a plant lectin, wheat-germ agglutinin 1994/01/02
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■ Academic conference presentation

1.	Culture study of animal cells using algae and animal-derived growth factor-producing cells （Speech，General） 2025/11/11
2.	Research on animal cell culture using algae extracts and culture supernatants of animal-derived growth factor-producing cells （Poster notice，General） 2025/08/29
3.	Environmental impacts of cultivated meat production in a circular cell culture system （Poster notice，General） 2024/11/19
4.	Utilization of Anabaena sp. extracts for bioactive substances （Poster notice，General） 2024/11/18
5.	Utilization of Anabaena sp. extracts for bioactive substances （Speech，General） 2024/10/10
6.	Potential application of microalgae in cultured medium for sustainable cultured meat production （Speech，General） 2023/10/16
7.	Circular cell culture system combining microalgae and mammalian cells for sustainable society （Speech，Panelist at Symposium/Workshop (Appointed)） 2023/03/25
8.	A novel culture medium for cultured meat production using microalgae-derived nutrients and mammalian cell-secreted growth factors: Development of serum-free and grain-derived nutrient-free medium （Poster notice，General） 2022/12/07
9.	Recycling culture system combining microalgae and mammalian cells for realization of sustainable society （Speech，Panelist at Symposium/Workshop (Appointed)） 2022/03/18
10.	Culture media using microalgae nutrients for sustainable culture meat production （Speech，Panelist at Symposium/Workshop (Appointed)） 2022/03/17
11.	Circular Food Production System Using Symbiotic Algae/Animal Cell （Speech，General） 2021/07/12
12.	Cell Cultivation Using Microalgal Extract for Production of Cultured Meat （Speech，General） 2019/10/07
13.	Contractile Force Measurement of Human iPS Cell-Derived Cardiac Cell Sheet-Tissues and Its Application to In Vitro Drug Testing （Poster notice，General） 2018/09/07
14.	Cell biological analysis of human iPS cell-derived beating cardiomyocytes in vitro and development of visible light-responsive collagen gel for cell purification （Poster notice，General） 2018/09/06
15.	Contractile Force Measurement of Human iPS Cell-Derived Myocardial Sheet for in Vitro Drug Testing （Speech，General） 2016/09/05
16.	Elimination of residual iPS cells in bioengineered cardiac cell sheets by TRPV-1 activation （Poster notice，General） 2016/06/23
17.	Contractile Force Measurement of Human iPS Cell-Derived Myocardial Sheet （Poster notice，General） 2015/09/09
18.	Cell-sheet-based bioengineered human cardiac tissue using pluripotent stem cells for heart repair and disease models （Poster notice，General） 2013/06/12
19.	The Functional Evaluation of ES Cell-derived Cardiac Cell Sheet with Different Types of Fibroblasts （Speech，General） 2013/03/23
20.	Expansion and cardiac differentiation of human iPS cells using a suspension culture system （Poster notice，General） 2012/09/07
21.	Creation of Cell Sheet-Based Bioengineered Heart Tissue using ES/iPS Cells-Derived Cardiomyocytes （Speech，General） 2012/03/16
22.	Electrical interaction between cardiomyocyte sheets separated by noncardiomyocyte sheet in heterogeneous tissues （Poster notice，General） 2009/08/30
23.	The electrophysiological analyses of layered cardiomyocyte sheets （Speech，General） 2005/07/06
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■ Research areas

Biomedical engineering, Cell biology

■ Academic society memberships

The Japanese Society for Regenerative Medicine

■ Education

1991/04～1995/03

〔Doctoral course〕〔Doctorial Course〕, Gunma University, Completed,