Faculty Information - KOBAYASHI Jiyun

Books

Published papers

Academic conference presentation

Conference activities & talks

Awards & honors

Education

Academic & professional experience

Rsearch funding number

External Researcher ID

（Last updated : 2025-03-25 08:59:54）

KOBAYASHI Jiyun
Department Graduate School of Medical Science, Graduate School of Medical Science

Position Assistant Professor

■ Books

1.	Chapter contribution	5.35 - Biomaterials: Temperature-Responsive Polymer, Comprehensive Biotechnology 2019/07
2.	Chapter contribution	Cell sheet technologies, Biomaterials Nanoarchitectonics 2016/02
3.	Chapter contribution	Sociocytology Illuminated by Reconstructing Functional Tissue with Cell Sheet Based Technology, Hyper Bio Assembler for 3D Cellular Systems 2015/07
4.	Chapter contribution	Micropatterning with a liquid crystal display (LCD) projector, Micropatterning in Cell Biology Part A 2014/02
5.	Chapter contribution	Thermoresponsive cell culture surfaces designed for cell-sheet-based tissue engineering and regenerative medicine, Biomaterials Surface Science 2013/09
6.	Chapter contribution	5.06 - Biomaterials: temperature-responsive polymer, Comprehensive Biotechnology 2012/09
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■ Published papers

1.	Review article	Molecular design of dynamically thermoresponsive biomaterials 2025/03/24
2.	Original article	Terminus-Selective Covalent Immobilization of Heparin on a Thermoresponsive Surface Using Click Chemistry for Efficient Binding of Basic Fibroblast Growth Factor. 2024/02
3.	Original article	Reproducible preparation of primary rat hepatocyte sheets using a thermoresponsive culture dish 2023/10/11
4.	Original article	Preservation of heparin-binding EGF-like growth factor activity on heparin-modified poly(N-isopropylacrylamide)-grafted surfaces 2021/11/18
5.	Review article	Cell sheet tissue engineering: Cell sheet preparation, harvesting/manipulation, and transplantation 2019/05
6.	Review article	Design of temperature-responsive polymer-grafted surfaces for cell sheet preparation and manipulation 2019/04
7.	Review article	Design of temperature-responsive cell culture surfaces for cell sheet-based regenerative therapy and 3D tissue fabrication 2018/10
8.	Original article	Effect of temperature changes on serum protein adsorption on thermoresponsive cell-culture surfaces monitored by a quartz crystal microbalance with dissipation 2018/05
9.	Review article	On-off affinity binding modulation on thermoresponsive polymer-grafted surfaces for capture and release of proteins and cells 2017/07
10.	Original article	Thermoresponsive anionic block copolymer brushes with a strongly anionic bottom segment for effective interactions with biomolecules 2016/09
11.	Original article	A heparin-modified thermoresponsive surface with heparin-binding epidermal growth factor-like growth factor for maintaining hepatic functions in vitro and harvesting hepatocyte sheets 2016/03
12.	Original article	Protein separations via thermally responsive ionic block copolymer brush layers 2016/03
13.	Original article	Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation 2015/08
14.	Original article	Measurement of the dynamic behavior of thin poly(N-isopropylacrylamide) hydrogels and their phase transition temperatures measured using reflectometric interference spectroscopy 2015/03
15.	Original article	Stripe-Patterned Thermo-responsive Cell Culture Dish for Cell Separation without Cell Labeling 2015/02
16.	Original article	Surface design of antibody-immobilized thermoresponsive cell culture dishes for recovering intact cells by low-temperature treatment 2014/11
17.	Original article	Thermoresponsive Anionic Copolymer Brushes Containing Strong Acid Moieties for Effective Separation of Basic Biomolecules and Proteins 2014/10
18.	Original article	Rate control of cell sheet recovery by incorporating hydrophilic pattern in thermoresponsive cell culture dish 2014/08
19.	Original article	Monolithic Silica Rods Grafted with Thermoresponsive Anionic Polymer Brushes for High-Speed Separation of Basic Biomolecules and Peptides 2014/04
20.	Original article	Thermoresponsive Copolymer Brushes Possessing Quaternary Amine Groups for Strong Anion-Exchange Chromatographic Matrices 2014/03
21.	Other	Selective cell adhesion and detachment on antibody-immobilized thermoresponsive surfaces by temperature changes 2013/08
22.	Review article	Heparin-functionalized thermoresponsive surface: A versatile cell culture substrate for regulating multivalent affinity binding with heparin-binding proteins by temperature changes 2013/07
23.	Review article	Thermoresponsive thin hydrogel-grafted surfaces for biomedical applications 2013/07
24.	Original article	Switching of cell growth/detachment on heparin-functionalized thermoresponsive surface for rapid cell sheet fabrication and manipulation 2013/06
25.	Original article	A molded hyaluronic acid gel as a micro-template for blood capillaries 2013/03
26.	Original article	Thermally Modulated Cationic Copolymer Brush on Monolithic Silica Rods for High-Speed Separation of Acidic Biomolecules 2013/02
27.	Original article	Modulation of graft architectures for enhancing hydrophobic interaction of biomolecules with thermoresponsive polymer-grafted surfaces 2012/11
28.	Original article	Thermally responsive microcarriers with optimal poly(N-isopropylacrylamide) grafted density for facilitating cell adhesion/detachment in suspension culture 2012/11
29.	Original article	Shear stress-dependent cell detachment from temperature-responsive cell culture surfaces in a microfluidic device 2012/10
30.	Original article	Simultaneous enhancement of cell proliferation and thermally induced harvest efficiency based on temperature-responsive cationic copolymer-grafted microcarriers 2012/06
31.	Other	Effect of Cell Density on Reproducibility in a Cell-Based Biosensor Using a Microwell Array 2012/05
32.	Original article	Temperature-responsive poly(N-isopropylacrylamide)-grafted microcarriers for large-scale non-invasive harvest of anchorage-dependent cells 2012/05
33.	Original article	High stability of thermoresponsive polymer-brush-grafted silica beads as chromatography matrices 2012/03
34.	Original article	Effect of reaction solvent on the preparation of thermo-responsive stationary phase through a surface initiated atom transfer radical polymerization 2011/12
35.	Original article	Thermoresponsive polymer brush on monolithic-silica-rod for the high-speed separation of bioactive compounds 2011/09
36.	Original article	Thermo-responsive protein adsorbing materials for purifying pharmaceutical protein on exposed charging surface 2011/02
37.	Original article	Thermally-modulated on/off-adsorption materials for pharmaceutical protein purification 2011/01
38.	Original article	Characterization of ultra-thin temperature-responsive polymer layer and its polymer thickness dependency on cell attachment/detachment properties 2010/10
39.	Original article	Development of microfabrication technology with maskless photolithography device using LCD projector 2010/10
40.	Original article	Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads by surface-initiated atom transfer radical polymerization for a highly resolutive separation of peptides 2010/09
41.	Original article	Effective separation of peptides using highly dense thermo-responsive polymer brush-grafted porous polystyrene beads 2010/08
42.	Original article	Hydration of poly(N-isopropylacrylamide) brushes on micro-silica beads measured by a fluorescent probe 2010/05
43.	Original article	Thermo-responsive polymer brush surfaces with hydrophobic groups for all-aqueous chromatography 2010/04
44.	Review article	Fabrication of a thermoresponsive cell culture dish: a key technology for cell sheet tissue engineering 2010/02
45.	Original article	Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules 2010/01
46.	Original article	Thermo-responsive polymer brush-grafted porous polystyrene beads for all-aqueous chromatography 2010/01
47.	Original article	Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing 2009/10
48.	Original article	Mass preparation of size-controlled mouse embryonic stem cell aggregates and induction of cardiac differentiation by cell patterning method 2009/09
49.	Review article	Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering 2009/06
50.	Original article	Temperature-responsive glass coverslips with an ultrathin poly(N-isopropylacrylamide) layer 2009/01
51.	Original article	Influence of graft interface polarity on hydration/dehydration of grafted thermoresponsive polymer brushes and steroid separation using all-aqueous chromatography 2008/10
52.	Original article	Effects of graft densities and chain lengths on separation of bioactive compounds by nanolayered thermoresponsive polymer brush surfaces 2008/01
53.	Original article	Cellular control of tissue architectures using a three-dimensional tissue fabrication technique. 2007/11
54.	Review article	Grand Espoir: Robotics in Regenerative Medicine 2007/10
55.	Original article	Interfacial property modulation of thermoresponsive polymer brush surfaces and their interaction with biomolecules 2007/08
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■ Academic conference presentation

1.	Creation and transplantation of angiogenic factor-secreting hepatocyte sheets by gene delivery 2024/12/19
2.	Visible light-induced covalent immobilization of poly(N-isopropylacrylamide) on polymeric substrate for thermoresponsive cell culture 2024/05/30
3.	Reproducible preparation of transplantable hepatic tissue sheets using thermoresponsive surfaces 2024/05/29
4.	Coating of visible light-crosslinkable poly(N-isopropylacrylamide) for thermoresponsive cell culture surfaces 2023/05/26
5.	Ex Vivo Gene Delivery for Fabrication of Hepatocyte Sheet Tissues Secreting Angiogenic Factors 2022/01/08
6.	Messenger RNA delivery for fabrication of hepatocyte tissues secreting angiogenic factors 2021/11/29
7.	Gene delivery for creation of hepatocyte sheets secreting angiogenic factors 2021/10/28
8.	Creation of transplantable hepatocyte sheet tissues by using thermoresponsive cell culture surfaces 2020/12/11
9.	Temperature-dependent binding of growth factors and cells to a heparin-immobilized thermoresponsive surface 2019/04/03
10.	Temperature-dependent affinity binding of heparin-binding growth factors and cells with a heparin-immobilized thermoresponsive surface 2018/09/05
11.	Design of thermoresponsive cell culture surfaces for creating hepatic tissues 2018/07/06
12.	Temperature-dependent affinity of heparin-binding growth factors and cells with a heparin-immobilized thermoresponsive surface 2018/06/19
13.	Dynamic analysis of thermoresponsive cell culture surfaces by quartz crystal microbalance with dissipation monitoring system 2017/11/10
14.	Heparin-immobilized thermoresponsive cell culture surfaces for sustained stimulation of heparin-binding EGF and recovery of cultured cell sheets by lowering temperature 2017/09/05
15.	Nano-intelligent surfaces for cell-sheet based regenerative medicine 2017/05/27
16.	Temperature-controlled capture and release of heparin-binding proteins and cells on heparin-immobilized thermoresponsive cell culture substrates 2017/04/05
17.	Temperature-dependent switching of growth factor binding on heparin-immobilized thermoresponsive surface for long-term stimulation of growth factorand recovery of cell sheets 2016/12/15
18.	ECM-mimicking thermoresponsive surface for manipulating hepatocyte sheets with maintenance of hepatic functions 2016/11/28
19.	Heparin-immobilized thermoresponsive cell culture surface fo sustained growth factor stimulation and cell sheet recovery 2016/11/28
20.	Heparin-immobilized thermoresponsive surface for manipulating hepatocyte sheets with maintenance of hepatic functions 2016/09/06
21.	Mimicking ECM nanostructure on thermoresponsive cell culture surfaces for creating functional cellular tissues 2016/07/27
22.	Evaluation of in vitro hepatocyte sheets on heparin-immobilized thermoresponsive cell culture surfaces 2016/05/26
23.	Hydrophobized thermoresponsive polymer brush on porous monolithic silica for effective bioseparations 2016/05/20
24.	Switching of growth factor binding on heparin-functionalized thermoresponsive surface for hepatocyte sheet manipulation with maintenance of hepatic functions 2016/05/18
25.	Thermoresponsive strong anionic copolymer brushes grafted silica beads for effective cation exchange chromatography 2015/12/17
26.	Fabrication of functional liver tissues by cell sheet-based bioassembler technologies 2015/11/24
27.	Effects of affinity-bound heparin-binding growth factors onto heparin-functionalized thermoresponsive cell culture surfaces on cultured cells 2015/09/17
28.	Creation of functional hepatocyte sheet tissues by using heparinized thermoresponsive surfaces 2015/09/09
29.	Thermoresponsive Anionic Copolymer Brush on Porous Monolithic Silica Rod For Separation of Basic Biomolecules 2015/08/31
30.	Thermoresponsive Anionic Copolymer Brushes having Strong Acid Group for Effective Separation of Basic Proteins 2015/08/18
31.	ECM-mimicking thermoresponsive cell culture surfaces for creation of hepatic tissues 2015/07/29
32.	Development of bioreactor for constructing three-dimensional tissue with vascularization 2015/07/21
33.	Heparin-functionalized thermoresponsive cell culture surfaces for creation of functional hepatic tissues 2015/07/09
34.	Efficient fabrication of hepatocyte cells sheet with heparin-immobilized temperature-responsive cell culture surfaces 2015/05/12
35.	Design of heparin-functionalized thermoresponsive cell culture surfaces for creation of hepatic tissues 2015/05/08
36.	Surface design of heparin-functionalized thermoresponsive cell culture substrates for maintaining hepatic functions and harvesting cultured hepatocyte sheets 2015/04/17
37.	Surface design of heparin-functionalized temperature-responsive cell culture surfaces for maintenance of cellular functions and cell sheet recovery 2014/12/03
38.	Thermoresponsive Copolymer Brushes Possessing Quaternary Amine Moieties for Effective Protein Adsorption Chromatography Matrices 2014/12/03
39.	Heparin-functionalized temperature-responsive cell culture surfaces for maintaining hepatic functions and harvesting cultured hepatocyte sheets 2014/10/08
40.	Thermoresponsive Cationic Copolymer Brush having Quaternary Amine Group for Effective Thermoresponsive Ion-exchange Chromatography 2014/10/08
41.	Surface design of advanced thermoresponsive cell culture surfaces for creating functional liver tissues 2014/09/10
42.	Silica Beads Grafted with Thermoresponsive Cationic Copolymer Brush Possessing Quaternary Amine Group for Effective Thermoresponsive Ion-exchange Chromatography 2014/09/01
43.	Thermoresponsive Anionic Copolymer Brush on Monolithic Silica for Effective Separation of Basic Biomolecules 2014/08/28
44.	Fabrication of adipose tissue-derived cells using charged thermoresponsive cell culture surfaces for enhancing effective transplantation 2014/05/29
45.	Fabrication of adipose tissue-derived cell sheets using charged thermoresponsive cell culture surfaces 2014/04/16
46.	Fabrication of hepatocyte sheets with maintenance of hepatic functions using heparin-functionalized thermoresponsive cell culture surface 2014/03/27
47.	Thermoresponsive Strong Cationic Copolymer Brushes Possessing Quaternary Amine Groups for Thermoresponsive Adsorption Chromatography 2014/03/24
48.	Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation 2013/12/03
49.	Heparin-functionalized thermoresponsive cell culture surfaces for regulating multivalent affinity binding with heparin-binding proteins by temperature changes 2013/10/25
50.	Heparin-functionalized thermoresponsive surfaces for the dynamic regulation of affinity interaction with heparin-binding proteins and cells 2013/10/08
51.	Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Label Free Cell Separation 2013/09/10
52.	Thermo-responsive Cationic Copolymer Brush Grafted Monolithic Silica Rod Columns for High-Speed Separation of Acidic Biomolecules 2013/08/29
53.	Design of heparin-functionalized thermoresponsive cell culture surfaces for rapid cell sheet formation and manipulation 2013/06/28
54.	Development of one-step fabrication method for cell micropatterning 2013/06/27
55.	Thermoresponsive Cationic Copolymer Brush Modified Monolithic Silica Rod for High-Speed Separation of Acidic Compounds 2013/06/27
56.	Thermoresponsive Cationic Copolymer Brush on Monolithic Silica Rods for High-Speed Separation of Acidic Biomolecules 2013/06/05
57.	Design of thermo-responsive cell culture surfaces for regulation of affinity binding by temperature changes 2013/06/04
58.	Heparin functionalized thermoresponsive cell culture surfaces for regulating affinity interaction with basic fibroblast growth factor and enhancing cell sheet formation 2013/04/12
59.	Biofunctional Thermo-Responsive Polymeric Surface with Micropatterns for Controlling Cell Attachment and Detachment 2013/04/10
60.	Surface design of thermoresponsive surfaces to regulate affinity binding between bFGF and immobilized heparin for rapid cell sheet fabrication 2013/03/19
61.	Preparation of thermo-responsive polymer brush on monolithic silica rod for high-speed analysis of bioactive compounds 2012/12/14
62.	Regulation of cell adhesion and detachment on antibody-immobilized surfaces using grafted thermoresponsive polymer 2012/12/13
63.	Thermoresponsive affinity interaction between cells and immobilized antibodies on poly(N-isopropylacrylamide)-grafted surfaces 2012/11/06
64.	Temperature-regulated affinity interaction between cells and the antibody-immobilized thermoresponsive thin hydrogel layer 2012/10/10
65.	Thermoresponsive polymer brush having hydrophobic basal layer for high stability of chromatographic matrices 2012/10/10
66.	Modulation of cell attachment and detachment on patterned thermo-responsive polymeric surface for cell separation 2012/09/08
67.	bFGF-immobilization on heparinized thermoresponsive cell culture substrate for enhancing cell proliferation 2012/09/05
68.	Temperature-regulated affinity interaction between cells and immobilized antibodies on thermoresponsive surfaces 2012/09/05
69.	Temperature-regulated affinity interaction between cells and immobilized antibodies on thermoresponsive surfaces 2012/07/23
70.	Cardiac Differentiation of Mouse Embryonic Stem Cells by Patterning Culture Method 2012/06/15
71.	Development of thermoresponsive surface for cell sheet harvest by photopolymerization 2012/06/04
72.	Regulation of affinity interaction between cells and immobilized antibodies using thermoresponsive polymer 2012/06/04
73.	Thermo-responsive Chromatography for Blood Proteins Purification 2012/06/03
74.	Regulation of affinity interaction between cells and the surface-immobilized antibody using thermoresponsive polymer 2012/05/30
75.	Affinity immobilization of bFGF on heparinized thermoresponsive cell culture substrate for enhancing cell growth 2012/03/17
76.	Modulation of cell attachment and detachment on thermo-responsive polymeric surface 2011/12/13
77.	Fabrication of thermoresponsive surface for cell sheet harvest by photopolymerization 2011/11/08
78.	Modulation of Cell Behavior on Thermo-Responsive Polymeric Surface with Hydrophilic Patterns 2011/09/14
79.	Thermo-responsive protein adsorbing materials for purifying pharmaceutical protein 2011/09/04
80.	Influence of immobilized basic fibroblast growth factor and fibronectin onto heparinized thermoresponsive cell culture substrates on cell proliferation 2011/05/25
81.	Thermoresponsive polymer brush modified monolithic silica for separating bioactive compounds 2011/05/22
82.	Antibody-immobilized thermoresponsive cell culture surfaces for promoting cell adhesion 2011/05/09
83.	Preparation of poly(N-isopropylacrylamide) brush grafted monolithic silica and characterization of them as chromatographic matrices 2011/05/09
84.	Immobilization of bFGF on heparinized thermoresponsive cell culture surface for enhancing cell growth 2011/04/13
85.	Thermoresponsive cell culture surfaces for promoting cell adhesion 2011/04/13
86.	Fast separation of bioactive compounds using thermo-responsive polymer grafted monolithic silica columns 2011/03/15
87.	Next-generation thermoresponsive cell culture surfaces for promoting cell adhesion 2011/03/15
88.	Enhancement of cell growth on heparinized thermoresponsive cell culture substrates with basic fibroblast growth factor 2011/02/13
89.	Preparation of thermo-responsive polymer brushes on hydrophilic polymeric beads for separating biomolecules with high resolution 2011/02/13
90.	Myocardial Differentiation of Mouse Embryonic Stem Cells by Cell-Patterning Method 2011/01/17
91.	Positively charged thermoresponsive cell culture surfaces for promoting cell adhesion 2010/12/15
92.	CARDIAC DIFFERENTIATION OF MOUSE ES CELLS BY PATTERNING CULTURE 2010/09/16
93.	CARDIAC DIFFERENTIATION OF MOUSE ES CELLS BY PATTERNING CULTURE 2010/09/15
94.	Preparation of Thermo-responsive Copolymer Brush Surfaces Including Hydrophobic Group and Characterization of Them as Chromatographic Matrices. 2010/05/26
95.	Cardiac differentiation of mouse ES cells by patterning culture. 2010/05/12
96.	Positively charged thermoresponsive cell culture dish for enhancing cell adhesion 2010/05/10
97.	Thermoresponsive Adsorption Materials for Protein Purification. 2010/05/10
98.	Micropatterning of hydrogels by visible light irradiation with maskless photolithography device 2010/04/21
99.	Micropatterning of hydrogels for vascular framework in stratified cell sheets 2009/08
100.	Rapid prototyping of micropatterned cells and microchannels by maskless photolithography 2009/06/27
101.	Surface micropatterning by visible light irradiation with the modified liquid crystal display projector 2009/05/27
102.	Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing 2009/05/20
103.	Surface micropatterning of hydrophilic polymers by visible light irradiation 2009/05/20
104.	Mobility of Poly(N-isopropylacrylamide) Brushes on Micro-Silica Beads 2009/05
105.	Thermoresponsive Anionic Copolymer Brush Surfaces for Adsorption Chromatography 2009/05
106.	Thermoresponsive Chromatography Using Polymer Brush Grafted Porous Polystyrene Beads 2009/05
107.	Maskless photolithography for surface patterning and microfluidic channel fabrication 2009/04/20
108.	Temperature-responsive chromatography for blood proteins separation 2009/02
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■ Conference activities & talks

1.	2022/09/13	Temperature-responsive cell culture surfaces for liver tissue engineering, Biomaterials Seminar (Tsukuba, Japan)
2.	2015/05/30	Creation of functional liver tissues by cell-sheet technologies, IEEE Robotics and Automaton Society (ICRA) 2015 Workshop "Hyper Bio Assembler for 3D Cellular System Innovation" (Seattle, USA)

■ Awards & honors

1.	2021/04	Kumagai Research Grant (Design of Biomaterials to deliver messenger RNA toward cell-sheet tissues)
2.	2015/05	Best Young Investigator Oral Presentation Award, The 5th Asian Biomaterials Congress (Design of heparin-functionalized thermoresponsive cell culture surfaces for creation of hepatic tissues)

■ Education

1.	2000/04～2003/03	〔Doctoral course〕 Department of Applied Chemistry, Graduate School, Division of Science and Engineering, Waseda University, Completed, Doctor of Engineering
2.	1998/04～2000/03	〔Master degree program〕 Department of Applied Chemistry, Graduate School, Division of Science and Engineering, Waseda University, Completed, M. Engineering
3.	1994/04～1998/03	Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Graduated, B. Engineering

■ Academic & professional experience

1.	2011/04～	Tokyo Women's Medical University Institute of Advanced Biomedical Engineering and Science Lecturer
2.	2007/04～2011/03	Tokyo Women's Medical University Institute of Advanced Biomedical Engineering and Science Assistant Professor
3.	2004/06～2007/03	Tokyo Women's Medical University Institute of Advanced Biomedical Engineering and Science Research Assistant
4.	2003/04～2004/05	Japan Science and Technology Corporation Researcher

■ Rsearch funding number

20385404

■ External Researcher ID

orcID

0000-0003-0493-6386