TAMURA Manabu    Department   Graduate School of Medical Science, Graduate School of Medical Science    Position   Associate Professor
Language	Japanese
Title	Intraoperative Flow Cytometry and MLPA Analysis of CUSA Specimens: Toward Clinical Use
Conference	the 43rd Annual Meeting of the Japan Society for Neuro-Oncology
Conference Type	Nationwide Conferences
Presentation Type	Poster notice
Lecture Type	General
Date	2025/12/07
Holding period	2025/12/07～2025/12/09
Society abstract	第43回日本脳腫瘍学会学術集会　プログラム・抄録集 135
Summary	PS12-4 Keyword: CUSA, iFC, MLPA Background:<BR> In brain tumor surgery, tissue aspirated by the Cavitron Ultrasonic Surgical Aspirator (CUSA) is often discarded despite containing abundant tumor cells. Intraoperative flow cytometry (iFC) and multiplex ligation-dependent probe amplification (MLPA) are useful for rapid cellular and molecular analyses, but the suitability of CUSA specimens remains unclear. Objective:<BR> This study aimed to establish an efficient method to collect CUSA specimens using the BoneDust Collector and to evaluate the clinical utility of iFC and MLPA by comparing CUSA samples with conventional tumor tissues from the same sites. Methods:<BR> Seventeen brain tumor patients were enrolled. CUSA aspirates were collected intraoperatively with the BoneDust Collector. Corresponding tumor tissues were obtained by standard methods. iFC assessed cell counts and DNA content in CUSA specimens. MLPA was performed on DNA extracted from frozen CUSA and conventional specimens to compare gene amplification profiles. Results:<BR> The BoneDust Collector enabled safe and stable collection of adequate CUSA samples. iFC showed comparable cell counts and DNA content between CUSA and conventional samples, with strong correlation in tumor cell proportion. MLPA analysis demonstrated good DNA quality from CUSA specimens, with gene amplification patterns and tumor content estimates consistent with conventional tissues. Conclusion:<BR> The BoneDust Collector allows efficient recovery of high-quality tumor cells from CUSA aspirates. Combining iFC and MLPA on these specimens provides reliable intraoperative cellular and molecular information, comparable to conventional tissues. These findings support the clinical application of CUSA specimens for rapid brain tumor diagnosis and molecular profiling. Future studies will focus on standardization and validation in larger cohorts.