ナカニシ トシオ   NAKANISHI Toshio
  中西 敏雄
   所属   医学部 医学科(東京女子医科大学病院)
   職種   特任教授
言語種別 日本語
発表タイトル モノクロタリンと低酸素環境によるラット肺高血圧モデルにおいて CXCR4と MSCマーカーに高発現が観察された
会議名 第54回日本小児循環器学会総会・学術集会
主催者 日本小児循環器学会
学会区分 全国規模の学会
発表形式 ポスター掲示
講演区分 一般
発表者・共同発表者◎張ていてい, 川口奈奈子, 羽山恵美子, 古谷喜幸, 中西敏雄
発表年月日 2018/07/07
開催地
(都市, 国名)
横浜市
概要 *ポスターセッション 45
心血管発生・基礎研究 2

Background: Pulmonary arterial hypertension (PAH) is characterized by a severe and fatal clinical syndrome. C-X-C chemokine receptor type 4 (CXCR4) is known to play a key role in recruiting mesenchymal stem cells (MSCs) from the bone marrow. CXCR4 inhibitors has been reported not only to inhibit tumor cell migration and proliferation, but also to ameliorate pulmonary arterial muscularization.

Purpose: Therefore, we established rat PAH models to investigate involvement of CXCR4 and MSCs in developing PAH. Methods: PAH in rats was induced by 5 weeks of chronic hypoxia and treatment with a single injection of monocrotaline (60 mg/kg). We measured the gene/protein expression levels of CXCR4 and stem cell/MSC markers, and then compared PAH experimental groups with controls.

Results: Successful establishment of the PAH models was confirmed by differences between PAH and control groups in right ventricular systolic pressure, Fulton index, and medial wall thickness as well as vascular occlusion score determined by immunohistochemical staining. Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) showed that the expression of CXCR4 SCF, c-Kit, and CD29, was significantly higher in the PAH group, which are known to be expressed in MSCs. Immunohistochemical staining also showed that the expression of CXCR4, c-Kit, and CD90 was significantly higher in the PAH group.

Conclusion: These results indicate that CXCR4 and MSCs may be involved in the pathogenesis of PAH. At the same time, stem cells may play an important role in pulmonary vascular remodeling.