MURAGAKI Yoshihiro    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Visiting Professor
Language	English
Title	Fully automatic rapid DNA Ploidy Analyzer for intraoperative rapid diagnosis support
Conference	35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society.
Presentation Type	Speech
Publisher and common publisher	◎SHIOYAMA Takahiro, SUZUKI Akane, NOMURA Kenichi,, SHIGAKI Naoki, KUBO Hirotsugu,, TAKEDA Sunako, YAMAMORI Shinji, MARUYAMA Takashi, ISEKI Hiroshi, KOMORI Takashi, NITTA Masayuki, SAKAYORI TAKASHI, MURAGAKI Yoshihiro
Date	2013/07
Venue (city and name of the country)	Osaka, Japan
Society abstract	Engineering in Medicine and Biology Society (EMBC), 2013 35th Annual International Conference of the IEEE 906-909
Summary	Abstract Frozen section studies are a useful method to rapidly define tumor malignancy and identify the extent of surgical resection. However, diagnosis with a frozen section is qualitative and sometimes difficult. Therefore a quantitative method for grading tumors is desired. We have already reported a technique of intraoperative flow cytometry (iFC) that supports intraoperative histopathological examination of frozen sections. In this study, we report an advanced system named "Fully Automatic Rapid DNA Ploidy Analyzer" with a tissue pretreatment function and a freeze-dried reagent kit for cell staining. To evaluate our system, we analyzed samples from glioma patients who underwent open surgery for brain tumors. We observed obvious difference of the Malignancy Index (MI) between neoplastic and perilesional brain tissue (26.0 ±22.1% and 4.1 ±2.5%, respectively, P<0.001). Cut-off level for identification of the tumor in the biopsy specimen was 6.8% which provided 86% sensitivity and 81% specificity. We also obtained a good correlation between the MI and histological grade (WHO grading). Our new system also enabled finishing the process from sample preparation to the end of analysis in ten minutes or less. These results demonstrate that our fully automatic rapid DNA ploidy analyzer is feasible for rapid determination of glioma presence in a surgical biopsy sample.