ホリ サヤカ   HORI Sayaka
  堀 沙耶香
   所属   医学部 医学科
   職種   講師
言語種別 日本語
発表タイトル Forkhead box transcription factors determine synaptic specificity of a strength-dependent sensory processing circuit
会議名 次世代脳冬のシンポジウム
学会区分 全国規模の学会
発表形式 ポスター掲示
講演区分 一般
発表者・共同発表者◎堀沙耶香, 三谷昌平
発表年月日 2018/12/13
開催地
(都市, 国名)
東京、日本
概要 Optimization of the types and timing of avoidance behaviors depending on the intensity of a noxious stimulus is essential for survival; however, processing in the central nervous system and its developmental bases are largely unknown. Here, we report that Caenorhabditis elegans preferentially selects one of three different types of avoidance behaviors depending on the strength of the noxious stimulus. We screened 279 neuronal transcription factors using a combination of optogenetics and RNA interference methods and identified 19 candidates required for avoidance behaviors. Two candidate genes, unc-130 (UNCoordinated 130), which encodes a human Forkhead box D4 (FOXD4) homolog, and fkh-9 (ForKHead transcription factor family 9) , which encodes an human Forkhead box G1 (FOXG1) homolog, are required for the neural fate determination of AIB interneurons and functions by regulating the expression of a major chemical synapse gene, namely, AMPA-type ionotropic glutamate receptor glr-1. However, both genes do not affect an electrical synapse gene, inx-1 (innexin 1), indicating that unc-130 and fkh-9 determine pre-synaptic specificity of sensory processing circuit depending on stimulus strength. In addition, it has been reported that unc-130/FOXD is regulated by the canonical Wnt signaling pathway in polarity determination. However, in our study, the canonical Wnt signaling pathway is not needed for optimization of avoidance behaviors depending on the intensity of a noxious stimulus. Our study will provide a novel molecular basis of the unc-130/FOXD and fkh-9/FOXG1 to specify the network for complex behavior.