ホリ サヤカ   HORI Sayaka
  堀 沙耶香
   所属   医学部 医学科
   職種   講師
言語種別 英語
発表タイトル Forkhead box transcription factors determine pre-synaptic specificity and synaptic transmission efficiency of a strength-dependent sensory processing circuit
会議名 Neuro2019
学会区分 全国規模の学会
発表形式 口頭
講演区分 シンポジウム・ワークショップ パネル(その他)
発表者・共同発表者Sayaka Hori, Shohei Mitani
発表年月日 2019/07/25
開催地
(都市, 国名)
Niigata, Japan
概要 Optimization of the types and timing of avoidance behaviors depending on the intensity of a noxious stimulus is essential for survival; however, processing in the central nervous system and its developmental bases are largely unknown. Here, we report that Caenorhabditis elegans preferentially selects one of three different types of avoidance behaviors depending on the strength of the noxious stimulus. We screened 279 neuronal transcription factors using a combination of optogenetics and RNA interference methods and identified 19 candidates required for avoidance behaviors. Two candidate genes, unc-130 (UNCoordinated 130), which encodes a human Forkhead box D4 (FOXD4) homolog, and fkh-9 (ForKHead transcription factor family 9), which encodes an human Forkhead box G1 (FOXG1) homolog, are required for the neural fate determination of the important interneurons for choice of avoidance behaviors, regulating the expression of a major chemical synapse gene, namely, an AMPA-type ionotropic glutamate receptor glr-1. However, both genes do not affect expression of a component of invertebrate electrical synapses, namely gap junctions, inx-1 (innexin 1). In addition, the double mutants show dramatic accumulation of RAB-3, which normally localizes to the synaptic vesicles, in the neurites of AIB interneurons. Artificial excitation of AIB interneurons by Channelrhodopsin-2 cannot induce proper avoidance behaviors in the mutants. Both results imply that the reduction of neurotransmitter release is associated with the RAB-3 accumulation. Our study will provide a novel molecular basis of the unc-130/FOXD and fkh-9/FOXG1 to specify the synaptic functions for complex behavior.
researchmap用URL https://researchmap.jp/horis/