タカダ タクマ   Takada Takuma
  髙田 卓磨
   所属   研究施設 研究施設
   職種   非常勤講師
言語種別 英語
発表タイトル The Novel Strategy for Fabricating Aligned Human iPS Cell-derived Cardiac Tissue Model for Disease Research and Drug Development
会議名 第84回日本循環器学会学術集会
学会区分 国際学会及び海外の学会
発表形式 ポスター掲示
講演区分 一般
発表者・共同発表者TAKADA Takuma, MATSUURA Katsuhisa, SASAKI Daisuke, SHIMIZU Tatsuya, Nobuhisa Hagiwara
発表年月日 2020/07/27
開催地
(都市, 国名)
Kyoto (Online)
概要 Introduction: Bioengineered human cardiac tissue has been developed worldwide as a tissue model. However, there are few reports on the development of aligned cardiac tissue model. Methods and Results: We created the micro V shaped surface with the height of 10µm and the interval of 30µm on silicon resin base materials. Molding fibrin gel on the V shaped silicon resin, the micro inversed V shaped surface were transcribed on the fibrin gel. After seeding human iPS cell-derived cardiomyocytes (hiPS-CM) on inversed V shaped fibrin gel (VFG), hiPS-CM aligned on VFG compared with hiPS-CM on normal fibrin gel (NFG) shown in upper Figure. Further, the directionality histogram showed each angle of actin filaments in hiPS-CM on VFG were more oriented in the same direction compared with those on NFG shown in lower Figure. Conclusions: Using this strategy, hiPS-CM might be oriented on fibrin gel. It might lead to develop more matured cardiac tissue model to investigate the contractile properties for disease research and drug development.