マサムネ　ケン   MASAMUNE Ken   正宗　賢    所属   医学研究科 医学研究科 (医学部医学科をご参照ください)    職種   教授
言語種別	英語
発表タイトル	Development of a Cell Isolation Device for Continuous Brain Tumor Resection Surgery Support System -Optimization of Roller Gap for Efficient Cell Isolation-
会議名	21st Asian Conference on Computer-Aided Surgery（ACCAS 2025）
学会区分	国際学会及び海外の学会
発表形式	口頭
講演区分	一般
発表者・共同発表者	◎SASAKI Ryoya, MASAMUNE Ken, HANAFUSA Akihiko, TAKAGI Motok
発表年月日	2025/09/29
国名	日本
開催地 （都市, 国名）	Fukuoka
開催期間	2025/09/28～2025/09/30
学会抄録	ACCAS2025 The 21st Asian Conference on Computer-Aided Surgery Proceedings 27-28
概要	To enable rapid cell dissociation for intraoperative brain tumor diagnosis, we developed a novel cell isolation device that generates shear stress between two high-speed rotating rollers. This study investigated the optimal roller gap to maximize dissociation efficiency, achieving single-cell suspensions from porcine brain tissue in approximately 30 s. Flow cytometry analysis revealed a maximum single-cell ratio of 98.47 士 0.72% at a peripheral roller velocity of 1.0 m/s with a 0.2 mm gap. These results define the optimal mechanical conditions for the device and support its utility in accelerating intraoperativediagnostic workflows. Keywords: Brain tumor surgery. Intraoperative rapid diagnosis, Cell isolation device