フジタ　キヨウヘイ   Fujita Kiyouhei   藤田　恭平    所属   医学部 医学科    職種   助教
言語種別	英語
発表タイトル	Characterizing and Modulating the Mechanical Properties of hydrogels from Ventricular Extracellular Matrix
会議名	10th Asian Control Conference 2015
学会区分	国際学会及び海外の学会
発表形式	口頭
講演区分	一般
発表者・共同発表者	◎Fujita Kyohei, Tsuchida Yuuki, Seki Hiroshi, Sato Daisuke, Nakumura Takao, Kosawada Tadashi, Feng Zhonggang, Shiraishi Yasuyuki, Umezu Mitsuo
発表年月日	2015/06/01
開催地 （都市, 国名）	Kota Kinabalu, Malaysia
開催期間	2015/05/31～2015/06/03
学会抄録	Proceedings of the 10th Asian Control Conference 2015 718-722 2015
概要	In order to differentiate pluripotent stem cells to cardiomyocytes, the most general method is to expose stem cells to various growth factors related to cardiogenesis. However, a novel method has been reported to induce cardiac differentiation of human ES cells without supplemental growth factors by culturing embryoid body of human ES cells in hybrid gels composed of cardiac extracellular matrix (ECM) and type I collagen. On the other hand, mechanical properties of scaffold is one of the critical cue for differentiation of stem cells. However, it has not been thoroughly investigated the mechanical properties of the scaffold made from cardiac ECM in view of this and other reports about the differentiation of stem cells into cardiomyocytes using cardiac ECM scaffold. In this study, we fabricated bio-hydrogels composed of goat ventricular extracellular matrix, and investigated the mechanical properties by means of uniaxial compression test. It showed that the ECM gels possess viscoelastic property. The elastic modulus K 1 in modified non-linear Kelvin model is 9.5 Pa for these gels and K 2 is 814.7 Pa. Moreover, we were able to improve the elastic moduli K 1 and K 2 up to 139.7 Pa and 2023.9 Pa, respectively, by chemical treatment using EDAC.