ササキ　ダイスケ   Sasaki Daisuke
  佐々木　大輔
   所属   医学研究科 医学研究科 (医学部医学科をご参照ください)
   職種   特任助教
Language English
Title The Novel Strategy for Fabricating Aligned Human iPS Cell-derived Cardiac Tissue Model for Disease Research and Drug Development
Conference 第84回日本循環器学会学術集会
Conference Type International society and overseas society
Presentation Type Poster notice
Lecture Type General
Publisher and common publisherTAKADA Takuma, MATSUURA Katsuhisa, SASAKI Daisuke, SHIMIZU Tatsuya, Nobuhisa Hagiwara
Date 2020/07/27
Venue
(city and name of the country)		 Kyoto (Online)
Summary Introduction: Bioengineered human cardiac tissue has been developed worldwide as a tissue model. However, there are few reports on the development of aligned cardiac tissue model. Methods and Results: We created the micro V shaped surface with the height of 10µm and the interval of 30µm on silicon resin base materials. Molding fibrin gel on the V shaped silicon resin, the micro inversed V shaped surface were transcribed on the fibrin gel. After seeding human iPS cell-derived cardiomyocytes (hiPS-CM) on inversed V shaped fibrin gel (VFG), hiPS-CM aligned on VFG compared with hiPS-CM on normal fibrin gel (NFG) shown in upper Figure. Further, the directionality histogram showed each angle of actin filaments in hiPS-CM on VFG were more oriented in the same direction compared with those on NFG shown in lower Figure. Conclusions: Using this strategy, hiPS-CM might be oriented on fibrin gel. It might lead to develop more matured cardiac tissue model to investigate the contractile properties for disease research and drug development.