イマムラ　ヤスタカ   IMAMURA Yasutaka   今村　泰崇    所属   医学部 医学科（東京女子医科大学病院）    職種   助教
Language	Japanese
Title	Relation between Effector Memory T Cells with Activation of Kv1.3 and Severity of Heart Failure
Conference	第83回日本循環器学会学術集会
Promoters	日本循環器学会
Conference Type	Nationwide Conferences
Presentation Type	Speech
Lecture Type	General
Publisher and common publisher	◎野村秀仁, 佐藤加代子, 鈴木敦, 今村泰崇, 松浦純也, 関口治樹, 坂井晶子, 志賀剛, 萩原誠久
Date	2019/03/31
Venue (city and name of the country)	横浜市
Summary	*Oral Presentation (Japanese)40 Heart Failure/Cardiomyopaty (Basic) Introduction: Inflammation is deeply involved in heart failure (HF). It is known that IFNγ levels of plasma were related with HF severity. However, it is not known the T cell immunology in HF. Hypothesis: We hypothesized that the patients with HF have the T cell related immunological disorder. Methods/Results: Ninety patients with HF (Age:65.6±15.2, Men:57.8%, LVEF:43.4±12.8%) were studied, and compared with 76 controls without HF (Cont). Inflammatory T cell cytokine, IL-6, IFNγ, and IL17, were increased in peripheral blood of HF (P<0.001, P<0.05, and P<0.001, respectively). Next, to investigate the T cell dysfunction in HF, we examine the memory T cell subsets by FACS. In HF, CD45RA-CCR7-effecter memory T cells (TEM) and CD45RA+CCR7-effecter T cells (TEMRA) were increased. To examine the effector function of T cells, we analyzed T cell phenotype. Activated IFNγ+CD4+Th1 cells, IFNγ+CD8+T cells, and perforin+granzyme+ CD8+Cytotoxic T cells (CTL) were increased in HF depending on the NYHA stages. Finally, the increased expression and current of Kv1.3 not but Kca3. 1 on CD4 and CD8 T cells depending on the NYHA stages, which has the effect of activation for TEM, were confirmed by FACS and patch clamp (P<0.01 and P<0.01, respectively). Conclusions: Activated TEM and Teff were elevated in HF patients. Cytotoxic CD4 and CD8 T cells with high expression of KV1.3 might have an important role for the outcome of HF patients.