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|5.06 - Biomaterials: temperature-responsive polymer
|Moo-Young Murray, Butler Michael, Webb Colin, Moreira Antonio, Grodzinski Bernard, Cui Z F, Agathos Spiros
|Kobayashi Jun, Akiyama Yoshikatsu, Yamato Masayuki, Okano Teruo
|Surface-modified temperature-responsive polymers are used as switching sequences to regulate interfacial phenomena and initiate unique biomedical and biotechnological applications. Special attention has been paid to poly(N-isopropylacrylamide) (PIPAAm), which exhibits a reversible temperature-dependent phase transition in aqueous solutions at the lower critical solution temperature (LCST) of 32 degree C. In this article, we focus on the properties and characterization of a PIPAAm-modified surface, which is a key technology for temperature-responsive chromatography and cell sheet-based tissue engineering. Temperature-responsive intelligent surfaces modified with PIPAAm exhibit hydrophilic/hydrophobic alteration in response to temperature change. Chromatographic separations are performed on several types of PIPAAm-modified surfaces in the aqueous mobile phase, and various effects related to the separation of bioactive compounds are discussed, including copolymer composition and graft polymer architecture. Additionally, cultured cells are harvested on temperature-responsive cell culture dishes by decreasing the temperature, without the use of digestive enzymes or chelating agents. Our group has developed cell sheet-based tissue engineering for therapeutic uses with single-layer or multilayered cell sheets recovered from temperature-responsive cell culture dishes. Using surface-derivation techniques, we developed a new generation of temperature-responsive cell culture dishes to accelerate the detachment of cell sheets and carriers to facilitate transferring and layering the cell sheets. We also developed a new methodology for constructing well-defined organs using microfabrication techniques.