AKAGIRI Satomi
   Department   Graduate School of Medical Science, Graduate School of Medical Science
   Position   Assistant Professor (Fixed Term)
Article types Original article
Language English
Peer review Peer reviewed
Title Inhalation of carbon monoxide ameliorates collagen-induced arthritis in mice and regulates the articular expression of IL-1beta and MCP-1.
Journal Formal name：Inflammation
Abbreviation：Inflammation
ISSN code：15732576/03603997
Domestic / ForeginForegin
Volume, Issue, Page 32(2),pp.83-88
Author and coauthor Takagi Tomohisa, Naito Yuji, Inoue Mamoru, Akagiri Satomi, Mizushima Katsura, Handa Osamu, Kokura Satoshi, Ichikawa Hiroshi, Yoshikawa Toshikazu
Publication date 2009/04
Summary Carbon monoxide (CO), long considered a toxic gas, has recently been shown to mediate anti-inflammatory effects in various animal models. The aim of this study was to investigate whether the inhalation of CO ameliorated collagen-induced arthritis (CIA) in mice. CIA was induced in female DBA/1 mice by the injection of an anti-type II collagen antibody and lipopolysaccharide. The CO treatment group was exposed to CO gas at a concentration of 200 ppm in a closed cage starting on the day of the injection with an anti-type II collagen antibody and throughout the remaining study period. The clinical arthritis scores was examined daily for swelling of the paws as a sign of arthritis. For histopathology, the sections of the hind legs were evaluated by hematoxylin-eosin staining. Moreover, we evaluated the expression of interleukin (IL)-1beta and monocyte chemoattractant protein-1 (MCP-1) mRNA in the hind paws. Both clinical arthritis scores as well as histological findings of joint inflammation were significantly reduced in mice treated with CO gas inhalation compared to untreated mice. Further, CO significantly inhibited the increased expression of IL-1beta and MCP-1 mRNA in paws at day 3 after the induction of arthritis. In conclusion, the inhalation of CO protected mice from the synovial inflammation of CIA. Based on these data, the beneficial effects of CO in murine RA model may be attributed to its anti-inflammatory properties.
DOI 10.1007/s10753-009-9106-6
PMID 19214726