TANABE Kenji    Department   Research Institutes and Facilities, Research Institutes and Facilities    Position   Associate Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Localization of SMAP2 to the TGN and its function in the regulation of TGN protein transport.
Journal	Formal name：Cell Structure and Function
Volume, Issue, Page	36,pp.83-95
Author and coauthor	Funaki, Tomo Kon, Shunsuke Ronn, Roger E Henmi, Yuji Kobayashi, Yuka Watanabe, Toshio Nakayama, Keiko Tanabe, Kenji Satake, Masanobu
Publication date	2011
Summary	SMAP2 is an Arf GTPase-activating protein that is located and functions on early endosome membranes. In the present study, the trans-Golgi network (TGN) was verified as an additional site of SMAP2 localization based on its co-localization with various TGN-marker proteins. Mutation of specific stretches of basic amino acid residues abolished the TGN-localization of SMAP2. Over-expression of wild-type SMAP2, but not of the mutated SMAP2, inhibited the transport of vesicular stomatitis virus-G protein from the TGN to the plasma membrane. In contrast, this transport was enhanced in SMAP2 (-/-) cells characterized by increased levels of the activated form of Arf. SMAP2 therefore belongs to an ArfGAP subtype that resides on the TGN and functions as a negative regulator of vesicle budding from the organelle.