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TAKAGI Riyou
Department Graduate School of Medical Science, Graduate School of Medical Science Position Assistant Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Dual-color FISH analyses of xenogeneic human fibroblast sheets transplanted to repair lung pleural defects in an immunocompromised rat model. |
| Journal | Formal name：BMC research notes Abbreviation：BMC Res Notes ISSN code：17560500/17560500 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 17(1),pp.139 |
| Author and coauthor | KANZAKI Masato†*, TAKAGI Ryo, MITSUBOSHI shota, SHIDEI Hiroaki, ISAKA Tamami, YAMATO Masayuki |
| Authorship | 2nd author |
| Publication date | 2024/05/15 |
| Summary | BACKGROUND:Pulmonary air leaks (PALs) due to visceral pleura injury during surgery is frequently observed after pulmonary resections and the complication is difficult to avoid in thoracic surgery. The development of postoperative PALs is the most common cause of prolonged hospitalization. Previously, we reported that PALs sealants using autologous dermal fibroblast sheets (DFSs) harvested from temperature-responsive culture dishes successfully closed intraoperative PALs during lung resection.OBJECTIVE:In this study, we investigated the fate of human DFSs xenogenetically transplanted onto lung surfaces to seal PALs of immunocompromised rat. Dual-color FISH analyses of human fibroblast was employed to detect transplantation human cells on the lung surface.RESULTS:One month after transplantation, FISH analyses revealed that transplanted human fibroblasts still composed a sheet-structure, and histology also showed that beneath the sheet's angiogenesis migrating into the sheets was observed from the recipient tissues. FISH analyses revealed that even at 3 months after transplantation, the transplanted human fibroblasts still remained in the sheet. Dual-color FISH analyses of the transplanted human fibroblasts were sparsely present as a result of the cells reaching the end of their lifespan, the cells producing extracellular matrix, and remained inside the cell sheet and did not invade the lungs of the host.CONCLUSIONS:DFS-transplanted human fibroblasts showed that they are retained within cell sheets and do not invade the lungs of the host. |
| DOI | 10.1186/s13104-024-06792-x |
| PMID | 38750547 |