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SEKINE Hidekazu
Department Graduate School of Medical Science, Graduate School of Medical Science Position Associate Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Densely vascularized thick 3D tissue shows enhanced protein secretion constructed with intermittent positive pressure. |
| Journal | Formal name：Communications biology Abbreviation：Commun Biol ISSN code：23993642/23993642 |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 8(1),pp.201 |
| Author and coauthor | KATSUURA Misako†, HOMMA Jun*, SEKINE Hidekazu*, SHIMIZU Tatsuya |
| Authorship | Corresponding author |
| Publication date | 2025/02/08 |
| Summary | Constructing a dense vascular endothelial network within engineered tissue is crucial for successful engraftment. The present study investigated the effects of air-compressing intermittent positive pressure (IPP) on co-cultured mesenchymal stem cells and vascular endothelial cells and evaluated the potential of IPP-cultured cell sheets for transplantation therapy. The results demonstrated that the IPP (+) group exhibited a denser vascular endothelial network and significantly increased cell sheet thickness compared to the IPP (-) group. Furthermore, in vivo experiments showed that IPP-cultured cell sheets enhanced the secretion of Gaussian luciferase by genetically modified mesenchymal stem cells. These findings highlight the IPP method as a technique that simultaneously enables the thickening of planar tissues and the construction of vascular networks. This approach demonstrates promise for fabricating functional, transplantable, and thick tissues with dense vascularization and a high capacity for protein secretion, paving the way for novel applications in regenerative medicine. |
| DOI | 10.1038/s42003-025-07627-6 |
| PMID | 39922952 |