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Tsukasa HARA
Department Research Institutes and Facilities, Research Institutes and Facilities Position Assistant Professor (Fixed Term) |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | Mitochondrial respiratory chain supercomplexes in human mononuclear leukocytes |
| Journal | Formal name：International Journal of Analytical Bio-Science Abbreviation：Int. J. Anal. BioSci. ISSN code：21877912/21877920 |
| Domestic / Foregin | Foregin |
| Publisher | (NPO)生物試料分析科学会 |
| Volume, Issue, Page | 4(1),pp.6-12 |
| Total page number | 7 |
| Author and coauthor | Hara Tsukasa, Amagai Ryosuke, Koga Michinobu, Okado-Matsumoto Ayako |
| Authorship | Lead author |
| Publication date | 2016/03 |
| Summary | An association of mitochondrial respiratory chain complexes is called a supercomplex. These supercomplexes enhance energy production and reduce electron leaking. Destabilization of supercomplexes may increase oxidative stress and mitochondrial dysfunction, and aging is a trigger for mitochondrial dysfunction. We used high-resolution clear native-polyacrylamide gel electrophoresis (hrCN-PAGE) and two-dimensional hrCN/SDS-PAGE to examine supercomplex formation in human mononuclear leukocytes and estimate complex I proportions for supercomplexes. The results were then compared in relation to sex and age group. Supercomplex clusters were more stable in females than in males. Larger supercomplexes were more stable among subjects aged 55–67 years than in those aged 21–25 years; smaller supercomplexes were less stable among subjects aged 55–67 years than in those aged 21–25 years. |
| Document No. | 2016296500 |