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論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Focused surface acoustic wave locally removes cells from culture surface.
掲載誌名	正式名：Lab on a chip 略　 称：Lab Chip ISSNコード：14730189/14730189
掲載区分	国外
巻・号・頁	21(7),pp.1299-1306
著者・共著者	INUI Takumi†, MEI Jiyang, IMASHIRO Chikahiro, KURASHINA Yuta, FRIEND James, TAKEMURA Kenjiro*
発行年月	2021/03
概要	Regenerative medicine and drug development require large numbers of high-quality cells, usually delivered from in vitro culturing. During culturing, the appearance of unwanted cells and an inability to remove them without damaging or losing most if not all the surrounding cells in the culture reduce the overall quality of the cultured cells. This is a key problem in cell culturing, as is the inability to sample cells from a culture as desired to verify the quality of the culture. Here, we report a method to locally remove cells from an adherent cell culture using a 100.4 MHz focused surface acoustic wave (SAW) device. After exposing a plated C2C12 mouse myoblast cell culture to phosphate buffered solution (PBS), ultrasound from the SAW device transmitted into the cell culture via a coupling water droplet serves to detach a small grouping of cells. The cells are removed from an area 6 × 10-3 mm2, equivalent to about 12 cells, using a SAW device-Petri dish water gap of 1.5 mm, a PBS immersion time of 300 s, and an input voltage of 75 V to the SAW device. Cells were released as desired 90% of the time, releasing the cells from the target area nine times out of ten runs. In the one trial in ten that fails, the cells partially release and remain attached due to inter-cellular binding. By making it possible to target and remove small groups of cells as desired, the quality of cell culturing may be significantly improved. The small group of cells may be considered a colony of iPS cells. This targeted cell removal method may facilitate sustainable, contamination-free, and automated refinement of cultured cells.
DOI	10.1039/d0lc01293a
PMID	33734243