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論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Formation of Large Scaffold-Free 3-D Aggregates in a Cell Culture Dish by Ultrasound Standing Wave Trapping.
掲載誌名	正式名：Ultrasound in medicine & biology 略　 称：Ultrasound Med Biol ISSNコード：1879291X/03015629
掲載区分	国外
巻・号・頁	45(5),pp.1306-1315
著者・共著者	Nakao Misa†, Imashiro Chikahiro, Kuribara Taiki, Kurashina Yuta, Totani Kiichiro, Takemura Kenjiro*
担当区分	2nd著者
発行年月	2019/05
概要	Cellular aggregates that mimic cell-cell interactions in vitro are essential for biological research. This study introduces a method to form large scaffold-free 3-D aggregates in a clinically ubiquitous cell culture dish using kilohertz-order ultrasound standing wave trapping (USWT). We fabricated an aggregate formation system in which a 60-mm dish was set above a Langevin transducer via water. The transducer was excited at 110.8 kHz, and then C2C12 myoblasts were injected into the dish and trapped at the node position of the standing wave. The diameter and thickness of the formed aggregate were 8 and 2.7 mm, respectively, which are larger than those of aggregates formed previously by USWT. Moreover, we confirmed that >94% of cells constituting the aggregates survived 9 h, and the protein expression of cells was not altered significantly. This method can be applied to form aggregates with high functionality, which contributes to the development of biological research methodology.
DOI	10.1016/j.ultrasmedbio.2019.01.013
PMID	30799124