モトハシ トモコ   Motohashi Tomoko
  本橋 智子
   所属   医学部 医学科
   職種   特任助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 An Aneuploidy-Free and Structurally Defined Balancer Chromosome Toolkit for Caenorhabditis elegans.
掲載誌名 正式名:Cell reports
略  称:Cell Rep
ISSNコード:(2211-1247)
掲載区分国外
巻・号・頁 22(1),232-241頁
著者・共著者 Dejima Katsufumi†, Hori Sayaka†, Iwata Satoru†, Suehiro Yuji, Yoshina Sawako, Motohashi Tomoko, Mitani Shohei*
発行年月 2018/01
概要 Balancer chromosomes are critical tools for genetic research. In C. elegans, reciprocal translocations that lead to aneuploidy have been widely used to maintain lethal and sterile mutations in stable stocks. Here, we generated a set of aneuploidy-free and structurally defined crossover suppressors that contain two overlapping inversions using the CRISPR/Cas9 system. The toolkit includes 13 crossover suppressors and covers approximately 63% of all C. elegans coding genes. Together with the classical intrachromosomal crossover suppressors, the system now covers 89% of the coding genes. We also labeled the created balancers with fluorescent and phenotypic markers. We show that the crossover suppressors are better for embryonic analysis compared with translocational balancers. Additionally, we demonstrate an efficient method to generate lethal alleles by targeting essential genes on a chromosome balanced with a crossover suppressor. The toolkit will allow more efficient experiments in which lethal and sterile mutants can be analyzed.
DOI 10.1016/j.celrep.2017.12.024
PMID 29298424