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ハギワラ ノブヒサ
Nobuhisa Hagiwara
萩原 誠久 所属 その他 その他 職種 非常勤嘱託 |
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| 論文種別 | 原著 |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome. |
| 掲載誌名 | 正式名：Circulation journal : official journal of the Japanese Circulation Society 略 称：Circ J ISSNコード：1346-9843/1347-4820 |
| 掲載区分 | 国内 |
| 出版社 | Japanese Circulation Society |
| 巻・号・頁 | 82(8),pp.2128-2135 |
| 著者・共著者 | KITAMURA Kazutaka†, SATO Kayoko*, SAWABE Motoji, YOSHIDA Masayuki, HAGIWARA Nobuhisa |
| 担当区分 | 最終著者 |
| 発行年月 | 2018/07 |
| 概要 | Background: Adhesion molecules have essential roles in the development of atherosclerosis. We investigated whether P-selectin glycoprotein ligand-1 (PSGL-1)-expressing CD4 T cells contribute to plaque instability in acute coronary syndrome (ACS).
Methods and Results: We studied the adhesion molecules on CD4 T cells from consecutive patients with ACS treated with thrombus-aspirating device and compared them with healthy controls (n=48 each). Blood, thrombi, and plaque samples from the culprit coronary arteries were collected by thrombus aspiration performed during emergency coronary artery angiography. According to flow cytometry results, peripheral CD4 T cells from ACS patients strongly expressed PSGL-1 and integrin β2 (P<0.05 for both) more than those from controls; culprit coronary arteries contained an abundance of PSGL-1+ (P<0.001) but not integrin β2+CD4 T cells. In addition, immunohistochemical analysis of the thrombus-aspirating device samples revealed numerous PSGL-1+CD4 T cells in plaques from the culprit lesions. Results from the selectin-binding assay demonstrated that activated PSGL-1+CD4 T cells from ACS patients bound to P- or E-selectin after triggering the T-cell receptor, and adhered to endothelial cells under laminar flow conditions (P<0.05 and P<0.05, respectively), inducing their apoptosis (P<0.01) via activated caspase-3, which correlated with PSGL-1 expression (R=0.788, P=0.021) and was suppressed by application of a PSGL-1-specific antibody (P<0.05). Conclusions: PSGL-1 contributed to cytotoxic CD4 T cell homing to the culprit coronary artery and promoted plaque instability in ACS. |
| DOI | 10.1253/circj.CJ-17-1270 |
| PMID | 29962384 |