オオヒガシ　セイジ   大東　誠司    所属   その他 その他    職種   非常勤嘱託
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Significant changes in the intestinal environment after surgery in patients with colorectal cancer.
掲載誌名	正式名：Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract 略　 称：J Gastrointest Surg ISSNコード：18734626/1091255X
掲載区分	国外
巻・号・頁	17(9),pp.1657-64
著者・共著者	Ohigashi Seiji †, Sudo Kazuki, Kobayashi Daiki, Takahashi Takuya, Nomoto Koji, Onodera Hisashi
発行年月	2013/09
概要	BACKGROUND:There have been very few detailed reports of the intestinal environment after surgical treatment for colorectal cancer (CRC). We analysed faecal microbiota, organic acids and pH to investigate the influence of colorectal surgery on the intestinal environment.METHODS:Faecal samples from 81 CRC patients were collected before the start of pre-operative preparation the day before surgery, as well as 7 days or more after surgery. Thirteen groups of intestinal microbiota, eight types of organic acids, and pH were measured using 16S rRNA-targeted reverse transcription-quantitative PCR, high-performance liquid chromatography and a pH meter, respectively.RESULTS:Total bacterial counts (10.3 ± 0.6 vs. 9.4 ± 1.2 log10 cells/g; p < 0.001) and the numbers of 6 groups of obligate anaerobes were significantly decreased after surgery. In contrast, the populations of Enterobacteriaceae, Enterococcus, Staphylococcus and Pseudomonas were significantly increased. Post-operatively, the concentration of total organic acids was lower (77.9 ± 40.1 vs. 50.1 ± 37.0 μmol/g; p < 0.001) than the pre-operative concentration, and a significant reduction in short-chain fatty acids (SCFAs) was observed.CONCLUSION:Significant changes in the intestinal environment, including marked decreases in obligate anaerobes, increases in pathogenic bacteria, and reductions in SCFAs, were detected after surgery for CRC.
DOI	10.1007/s11605-013-2270-x
PMID	23807702