Fujiko Tsukahara    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Hsc70 regulates the nuclear export but not the import of influenza viral RNP: A possible target for the development of anti-influenza virus drugs.
Journal	Formal name：Drug discoveries & therapeutics Abbreviation：Drug Discov Ther ISSN code：1881-7831/1881-7831
Domestic / Foregin	Foregin
Volume, Issue, Page	2(2),pp.77-84
Author and coauthor	Watanabe K, Takizawa N, Noda S, Tsukahara F, Maru Y, Kobayashi N
Publication date	2008/04
Summary	In our previous report, we demonstrated that the matrix 1 (M1) protein of influenza virus directly binds to heat shock cognate protein 70 (Hsc70). The down-regulation of Hsc70 resulted in the reduction of influenza virus production, thus suggesting that Hsc70 plays a crucial role for viral replication. However, the detailed role of Hsc70 in viral replication remains to be elucidated. Hsc70 has been suggested to play a significant role in both the nuclear import and export processes. In this report, using leptomycin B (LMB), a CRM1-mediated nuclear export inhibitor, we demonstrated that Hsc70 forms a complex with vRNP through M1 in infected cells and in the virion, thus playing a significant role in the export of vRNP from the nucleus but not in the import of vRNP into the nucleus. The regulation of Hsc70 may therefore lead to the development of new anti-influenza virus drugs without raising mutant viruses.
PMID	22504503