菅野　仁    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	p53 gene mutation in 150 dissected lymph nodes in a patient with esophageal cancer.
Journal	Formal name：Diseases of the esophagus : official journal of the International Society for Diseases of the Esophagus / I.S.D.E Abbreviation：Dis Esophagus ISSN code：1120-8694(Print)1120-8694(Linking)
Volume, Issue, Page	11(4),pp.279-83
Author and coauthor	Kajiyama Y, Kanno H, Ueno M, Udagawa H, Tsutsumi K, Kinoshita Y, Nakamura T, Akiyama H, Miwa S, Tsurumaru M
Authorship	2nd author
Publication date	1998/10
Summary	For thoracic esophageal cancer, we perform extended three field lymph node dissection, and have achieved nearly 50% of overall 5-year survival. However, patients sometimes develop lymph node recurrences in spite of having no lymph node metastases found by conventional histopathologic examination. In a patient with esophageal squamous cell carcinoma, we sequenced all the p53 cDNA translated regions (exon 2-10) of primary carcinoma, and confirmed one p53 nonsense mutation in exon 10. Then we extracted genomic DNA from 150 surgically dissected lymph nodes from that patient, and performed polymerase chain reaction analysis (PCR-RFLP) to detect the same p53 mutation in the lymph nodes. PCR-RFLP analysis showed the same p53 mutation in six lymph nodes. One node was located along the right recurrent laryngeal nerve, where no positive nodes was identified by conventional histopathologic examination. The p53 mutational diagnosis of metastatic cancer may be useful in detecting minimal residual disease.
Document No.	10071815