菅野　仁    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Upregulation of tumor necrosis factor receptor 1 and TNF-alpha converting enzyme during corneal wound healing.
Journal	Formal name：Japanese journal of ophthalmology Abbreviation：Jpn J Ophthalmol ISSN code：0021-5155(Print)0021-5155(Linking)
Volume, Issue, Page	52(5),pp.393-8
Author and coauthor	Sakimoto Tohru, Yamada Ai, Kanno Hitoshi, Sawa Mitsuru
Publication date	2011/12
Summary	PURPOSE:To elucidate tumor necrosis factor (TNF) receptor-1 (TNFR1) and TNF-alpha converting enzyme (TACE) involvement during corneal wound healing.METHODS:The corneas of BALB/c mice cornea were scarred by alkali burns using filter paper dipped in 1N NaOH solution. TACE and TNFR1 expression in the alkali-burned corneas were evaluated by immunohistochemistry (total, 16 eyes). Using cultured fibroblasts (human) and macrophages (mice), we evaluated the release of soluble TNF-alpha (sTNF-alpha) and soluble TNFR1 (sTNFR1) in the supernatant by enzyme-linked immunosorbent assay after stimulating TACE activity with phorbol myristate acetate (PMA).RESULTS:In alkali-burned corneas, both TACE and TNFR1 expression were observed in the stromal cells after the acute phase of wound healing response. In macrophage-cultured supernatant, both sTNF-alpha and sTNFR1 release were promoted by PMA stimulation. On the other hand, only sTNFR1 released by PMA stimulation was observed in fibroblast-cultured supernatant.CONCLUSIONS:TACE and TNFR1 were expressed mainly after the acute phase of corneal wound healing. The TACE-dependent extracellular release of sTNFR1 was recognized in cultured fibroblasts and macrCONCLUSIONS:ophages.
DOI	10.1007/s10384-008-0536-8
Document No.	18991041