カトウ ヒデヒト   Kato Hidehito
  加藤 秀人
   所属   医学部 医学科
   職種   助教
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 High affinity of interaction between superantigen and TCR Vβ molecules induces a high level and prolonged expansion of superantigen-reactive CD4+ T cells.
掲載誌名 正式名:The Journal of biological chemistry
略  称:J Biol Chem
ISSNコード:00219258/1083351X
巻・号・頁 285(40),pp.30427-30435
著者・共著者 OMOE Katsuhiko† ,NUNOMURA Wataru ,KATO Hidehito ,LI Zhong-Juan ,IGARASHI Osamu ,ARAAKE Minako ,SANO Keigo ,ONO Hisaya K ,ABE Yohei ,HU Dong-Liang ,NAKANE Akio ,KIYONO Hiroshi ,TAKAKUWA Yuichi ,SHINAGAWA Kunihiro ,UCHIYAMA Takehiko ,IMANISHI Ken'ichi*
発行年月 2010/10
概要 In mice implanted with an osmotic pump filled with the superantigen (SAG) staphylococcal enterotoxin A (SEA), the Vbeta3+CD4+ T cells exhibited a high level of expansion whereas the Vbeta11+CD4+ T cells exhibited a mild level of expansion. In contrast, in mice implanted with an osmotic pump filled with SE-like type P (SElP, 78.1% homologous with SEA), the Vbeta11+CD4+ T cells exhibited a high level of expansion while the Vbeta3+CD4+ T cells exhibited a low level of expansion, suggesting that the level of the SAG-induced response is determined by the affinities between the TCR Vbeta molecules and SAG. Analyses using several hybrids of SEA and SElP showed that residue 206 of SEA determines the response levels of Vbeta3+CD4+ and Vbeta11+CD4+ T cells both in vitro and in vivo. Analyses using the above-mentioned hybrids showed that the binding affinities between SEA and the Vbeta3/Vbeta11 beta chains and between SEA-MHC class II-molecule complex and Vbeta3+/Vbeta11+ CD4+ T cells determines the response levels of the SAG-reactive T cells both in vitro and in vivo.