ミタニ シヨウヘイ
MITANI Shohei
三谷 昌平 所属 医学部 医学科 職種 教授・基幹分野長 |
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論文種別 | 原著 |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Structure and function analysis of the C. elegans aminophospholipid translocase TAT-1. |
掲載誌名 | 正式名:Journal of cell science 略 称:J Cell Sci ISSNコード:(1477-9137)0021-9533(Linking) |
掲載区分 | 国外 |
巻・号・頁 | 132(5),pp.1-7 |
著者・共著者 | Chen Yu-Zen, Klöditz Katharina, Lee Eui-Seung, Nguyen Diemmy Pham, Yuan Quan, Johnson Jack, Lee-Yow Yannick, Hall Adam, Mitani Shohei, Xia Ning-Shao, Fadeel Bengt, Xue Ding |
発行年月 | 2019/02 |
概要 | The Caenorhabditis elegans aminophospholipid translocase TAT-1 maintains phosphatidylserine (PS) asymmetry in the plasma membrane and regulates endocytic transport. Despite these important functions, the structure-function relationship of this protein is poorly understood. Taking advantage of the tat-1 mutations identified by the C. elegans million mutation project, we investigated the effects of 16 single amino acid substitutions on the two functions of the TAT-1 protein. Two substitutions that alter a highly conserved PISL motif in the fourth transmembrane domain and a highly conserved DKTGT phosphorylation motif, respectively, disrupt both functions of TAT-1, leading to a vesicular gut defect and ectopic PS exposure on the cell surface, whereas most other substitutions across the TAT-1 protein, often predicted to be deleterious by bioinformatics programs, do not affect the functions of TAT-1. These results provide in vivo evidence for the importance of the PISL and DKTGT motifs in P4-type ATPases and improve our understanding of the structure-function relationship of TAT-1. Our study also provides an example of how the C. elegans million mutation project helps decipher the structure, functions, and mechanisms of action of important genes. |
DOI | 10.1242/jcs.227660 |
PMID | 30683797 |