ミタニ シヨウヘイ   MITANI Shohei
  三谷 昌平
   所属   医学部 医学科
   職種   教授・講座主任
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 The small GTPase ARF-1.2 is a regulator of unicellular tube formation in Caenorhabditis elegans.
掲載誌名 正式名:The journal of physiological sciences : JPS
略  称:J Physiol Sci
ISSNコード:(1880-6562)1880-6546(Linking)
掲載区分国内
巻・号・頁 69(1),47-56頁
著者・共著者 Kage-Nakadai Eriko, Sun Simo, Iwata Satoru, Yoshina Sawako, Nishikawa Yoshikazu, Mitani Shohei
発行年月 2019/01
概要 The membrane trafficking events that regulate unicellular tube formation and maintenance are not well understood. Here, using an RNAi screen, we identified the small GTPase ARF1 homolog ARF-1.2 as a regulator of excretory tube formation in Caenorhabditis elegans. RNAi-mediated knockdown and knockout of the arf-1.2 gene resulted in the formation of large intracellular vacuoles at the growth sites (varicosities) of the excretory canals. arf-1.2 mutant animals were sensitive to hyperosmotic conditions. arf-1.2 RNAi affected the localization of the anion transporter SULP-8, which is expressed in the basal plasma membrane of the excretory canals, but did not affect the expression of SULP-4, which is expressed in the apical membrane. The phenotype of arf-1.2 mutants was suppressed by mutation of the small Rho GTPase CDC-42, a regulator of apical/basal traffic balance. These results suggest that ARF-1.2 plays an essential role in basal membrane traffic to regulate the formation of the unicellular excretory tube.
DOI 10.1007/s12576-018-0617-5
PMID 29704149