ミタニ シヨウヘイ   MITANI Shohei
  三谷 昌平
   所属   医学部 医学科
   職種   教授・講座主任
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 GOP-1 promotes apoptotic cell degradation by activating the small GTPase Rab2 in C. elegans.
掲載誌名 正式名:The Journal of cell biology
略  称:J Cell Biol
ISSNコード:(1540-8140)0021-9525(Linking)
掲載区分国外
巻・号・頁 216(6),1775-1794頁
著者・共著者 Yin Jianhua, Huang Yaling, Guo Pengfei, Hu Siqi, Yoshina Sawako, Xuan Nan, Gan Qiwen, Mitani Shohei, Yang Chonglin, Wang Xiaochen
発行年月 2017/06
概要 Apoptotic cells generated by programmed cell death are engulfed by phagocytes and enclosed within plasma membrane-derived phagosomes. Maturation of phagosomes involves a series of membrane-remodeling events that are governed by the sequential actions of Rab GTPases and lead to formation of phagolysosomes, where cell corpses are degraded. Here we identified gop-1 as a novel regulator of apoptotic cell clearance in Caenorhabditis elegans Loss of gop-1 affects phagosome maturation through the RAB-5-positive stage, causing defects in phagosome acidification and phagolysosome formation, phenotypes identical to and unaffected by loss of unc-108, the C. elegans Rab2 GOP-1 transiently associates with cell corpse-containing phagosomes, and loss of its function abrogates phagosomal association of UNC-108. GOP-1 interacts with GDP-bound and nucleotide-free UNC-108/Rab2, disrupts GDI-UNC-108 complexes, and promotes activation and membrane recruitment of UNC-108/Rab2 in vitro. Loss of gop-1 also abolishes association of UNC-108 with endosomes, causing defects in endosome and dense core vesicle maturation. Thus, GOP-1 is an activator of UNC-108/Rab2 in multiple processes.
DOI 10.1083/jcb.201610001
PMID 28424218