HASHIMOTO kazunori    Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine    Position   Professor (Fixed Term)
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Possible Application of Ascites-infiltrating Gamma-delta T Cells for Adoptive Immunotherapy.
Journal	Formal name：Anticancer research Abbreviation：Anticancer Res ISSN code：17917530/02507005
Domestic / Foregin	Foregin
Volume, Issue, Page	38(7),pp.4327-4331
Author and coauthor	Abe Yuki†, Kobayashi Hirohito*, Akizawa Yoshika, Ishitani Ken, Hashimoto Kazunori, Matsui Hideo
Publication date	2018/07
Summary	BACKGROUND/AIM:Malignant ascites contain many tumour-infiltrating lymphocytes. γδ T cells with antitumour activity have attracted attention as effector cells in cancer immunotherapy. Vδ2+ T cells were cultured from peripheral blood mononuclear cells (PBMCs) and ascites-infiltrating lymphocytes (AILs) to compare the differences in response to 2-methyl-3-butenyl-1-pyrophosphate (2M3B1-PP) and zoledronate (Zol) as antigens in vitro.MATERIALS AND METHODS:To expand Vδ2+ T cells from PBMCs and AILs from 29 patients with cancer, these cells were cultured and subjected to analysis.RESULTS:The proliferation rate of Vδ2+ T cells was higher in both PBMCs and AILs when cultured with Zol than with 2M3B1-PP. Although Vδ2+ T cells show a higher rate of expansion in AILs compared to PBMCs, the number of mixed tumour cells in ascites was decreased when cultured with Zol.CONCLUSION:Vδ2+ T cells in AILs are cytotoxic to tumour cells in ascites and may be considered in adoptive immunotherapy.
DOI	10.21873/anticanres.12732
PMID	29970569