ICHIHARA Atsuhiro    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor and Division head
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Novel sandwich ELISA for detecting the human soluble (pro)renin receptor.
Journal	Formal name：Frontiers in bioscience (Elite edition) Abbreviation：Front Biosci (Elite Ed) ISSN code：1945-0508(Electronic)1945-0494(Linking)
Volume, Issue, Page	5,pp.583-90
Author and coauthor	MARUYAMA Nobuhiro†, SEGAWA Tatsuya, KINOSHITA Noriaki, ICHIHARA Atsuhiro
Publication date	2013/01
Summary	The (pro)renin receptor ((P)RR) plays a key role in the activation of the local renin-angiotensin system via interaction with renin and prorenin. A truncated form that is cleaved by furin, referred to as soluble (pro)renin receptor (s(P)RR), is secreted into the extracellular space. An accurate measurement of s(P)RR levels in vivo is an important issue in elucidating the roles of (P)RR in physiology and pathophysiology. To address this, we developed a sandwich ELISA that is applicable to human subjects. The standard curve of this ELISA showed a high linearity (125-8,000 pg/ml) with a correlation coefficient of>0.99. The recovery rate was approximately 90% in human blood and urine samples. The s(P)RR levels in plasma of healthy volunteers was in the range from 15.2 to 35.1 ng/ml (n = 20). Intra- and inter-assay coefficient of variations were less than 5.5% and 7.5%, respectively. It thus appears that this ELISA is a reliable tool for measuring s(P)RR levels in human subjects.
Document No.	23277014