Kikuchi Ken    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli.
Journal	Formal name：Japanese journal of infectious diseases Abbreviation：Jpn J Infect Dis ISSN code：18842836/13446304
Domestic / Foregin	Domestic
Volume, Issue, Page	72(2),pp.81-87
Author and coauthor	Kabir S M Lutful, Chowdhury Nityananda, Asakura Masahiro, Shiramaru Sachi, Kikuchi Ken, Hinenoya Atsushi, Neogi Sucharit Basu, Yamasaki Shinji
Publication date	2019/03
Summary	Proper surveillance of Campylobacter jejuni and Campylobacter coli, major pathogens associated with human gastroenteritis, is necessary to tackle the increasing disease burden. To detect these pathogenic species, a variety of PCR assays have been developed. This study examined the sensitivity and specificity of 12 PCR assays targeting 23S rRNA, ceuE, lpxA, hipO, mapA, ask, and cdt genes of C. jejuni and C. coli. The sensitivities of PCR assays were 85.2-100%, and 97-100%, and the specificities were 90.5-100%, and 94.3-100% for the tested C. jejuni (n = 61) and C. coli (n = 33) strains, respectively. Two PCR assays, targeting cdtC and hipO genes, were found to be 100% sensitive and/or specific for all C. jejuni strains, while 3 assays, targeting cdtB, cdtA, and ask genes, were 100% sensitive and/or specific for C. coli strains. However, PCR assays for hipO and ask genes are problematic to conduct simultaneously due to the differences in PCR conditions. Overall, multiplex PCR assays targeting cdtC and cdtB genes, encoding 2 subunits of the same toxin, were concluded to be the most reliable. The results of this study would aid in proper surveillance of C. jejuni and C. coli and adopting intervention strategies in the near future.
DOI	10.7883/yoken.JJID.2018.340
PMID	30381691