キクチ　ケン   Kikuchi Ken   菊池　賢    所属   医学部 医学科（東京女子医科大学病院）    職種   教授
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli.
掲載誌名	正式名：Japanese journal of infectious diseases 略　 称：Jpn J Infect Dis ISSNコード：18842836/13446304
掲載区分	国内
巻・号・頁	72(2),pp.81-87
著者・共著者	Kabir S M Lutful, Chowdhury Nityananda, Asakura Masahiro, Shiramaru Sachi, Kikuchi Ken, Hinenoya Atsushi, Neogi Sucharit Basu, Yamasaki Shinji
発行年月	2019/03
概要	Proper surveillance of Campylobacter jejuni and Campylobacter coli, major pathogens associated with human gastroenteritis, is necessary to tackle the increasing disease burden. To detect these pathogenic species, a variety of PCR assays have been developed. This study examined the sensitivity and specificity of 12 PCR assays targeting 23S rRNA, ceuE, lpxA, hipO, mapA, ask, and cdt genes of C. jejuni and C. coli. The sensitivities of PCR assays were 85.2-100%, and 97-100%, and the specificities were 90.5-100%, and 94.3-100% for the tested C. jejuni (n = 61) and C. coli (n = 33) strains, respectively. Two PCR assays, targeting cdtC and hipO genes, were found to be 100% sensitive and/or specific for all C. jejuni strains, while 3 assays, targeting cdtB, cdtA, and ask genes, were 100% sensitive and/or specific for C. coli strains. However, PCR assays for hipO and ask genes are problematic to conduct simultaneously due to the differences in PCR conditions. Overall, multiplex PCR assays targeting cdtC and cdtB genes, encoding 2 subunits of the same toxin, were concluded to be the most reliable. The results of this study would aid in proper surveillance of C. jejuni and C. coli and adopting intervention strategies in the near future.
DOI	10.7883/yoken.JJID.2018.340
PMID	30381691