キクチ ケン   Kikuchi Ken
  菊池 賢
   所属   医学部 医学科(東京女子医科大学病院)
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読有り
表題 Potential Impact of Rapid Blood Culture Testing for Gram-Positive Bacteremia in Japan with the Verigene Gram-Positive Blood Culture Test.
掲載誌名 正式名:The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale
略  称:Can J Infect Dis Med Microbiol
ISSNコード:(1712-9532)1712-9532(Linking)
掲載区分国外
巻・号・頁 2017,4896791頁
著者・共著者 Kikuchi Ken, Matsuda Mari, Iguchi Shigekazu, Mizutani Tomonori, Hiramatsu Keiichi, Tega-Ishii Michiru, Sansaka Kaori, Negishi Kenta, Shimada Kimie, Umemura Jun, Notake Shigeyuki, Yanagisawa Hideji, Takahashi Hiroshi, Yabusaki Reiko, Araoka Hideki, Yoneyama Akiko
発行年月 2017/02
概要 Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan. Methods. A total 347 BC-GP assays were performed on clinical and simulated blood cultures. BC-GP results were compared to results obtained by reference methods for genus/species identification and detection of resistance genes using molecular and MALDI-TOF MS methodologies. Results. For identification and detection of resistance genes at two clinical sites and simulated blood cultures, overall concordance of BC-GP with reference methods was 327/347 (94%). The time for identification and antimicrobial resistance detection by BC-GP was significantly shorter compared to routine testing especially at the cardiology hospital, which does not offer clinical microbiology services on weekends and holidays. Conclusion. BC-GP generated accurate identification and detection of resistance markers compared with routine laboratory methods for Gram-positive organisms in specialized clinical settings providing more rapid results than current routine testing.
DOI 10.1155/2017/4896791
PMID 28316631