キクチ ケン   Kikuchi Ken
  菊池 賢
   所属   医学部 医学科(東京女子医科大学病院)
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読有り
表題 Development of an appropriate PCR system for the reclassification of Streptococcus suis.
掲載誌名 正式名:Journal of microbiological methods
略  称:J Microbiol Methods
ISSNコード:(1872-8359)0167-7012(Linking)
掲載区分国外
巻・号・頁 107,66-70頁
著者・共著者 Ishida Sakura, Tien Le Hong Thuy, Osawa Ro, Tohya Mari, Nomoto Ryohei, Kawamura Yoshiaki, Takahashi Tatsuhumi, Kikuchi Naoya, Kikuchi Ken, Sekizaki Tsutomu
発行年月 2014/12
概要 Thirty-five serotypes of Streptococcus suis (serotypes 1-34 and serotype 1/2) have so far been described on the basis of their polysaccharide capsular antigens. However, in the last decade, some serotype reference strains have been reexamined for their taxonomic status, and the reference strains of serotypes 20, 22, 26, 32, 33, and 34 may be different from taxon S. suis. In the present study, we developed a novel PCR method targeting the recombination/repair protein (recN) gene of S. suis, designated recN PCR, which corresponds to the current reclassification of this bacterium. We compared its specificity with other PCR methods for S. suis, and the results obtained confirmed its specificity. In addition, the detection limits of recN PCR were similar among all the reference strains of authentic S. suis, indicating that the recN PCR gave reliable results against bacterial strains and isolates used in this study. Therefore, recN PCR described in the present study will be a useful tool for the identification of authentic S. suis, and can also be used in epidemiological studies on this bacterium.
DOI 10.1016/j.mimet.2014.09.003
PMID 25229648