ミツイシ ツヨシ
  三石 剛
   所属   医学部 医学科(附属八千代医療センター)
   職種   准教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Molecular cloning and characterisation of a novel type of human papillomavirus 160 isolated from a flat wart of an immunocompetent patient.
掲載誌名 正式名:PloS one
略  称:PLoS One
ISSNコード:1932620319326203
掲載区分国外
巻・号・頁 8(11),e79592頁
著者・共著者 Mitsuishi Tsuyoshi, Ohsawa Ikuroh, Kato Toshihiko, Egawa Nagayasu, Kiyono Tohru
発行年月 2013
概要 More than 150 types of Human papillomaviruses (HPVs) have been isolated from numerous cutaneous and/or mucosal lesions. Flat wart samples on the face from 36 immunocompetent patients were collected and screened for HPV. From one sample, we cloned a putative novel genotype. The novel type consisted of 7779 bp in length with a GC content of 47.1%, containing open reading frames for putative early proteins (E1, E2, E4, E6, and E7) and two late proteins (L1 and L2). Homology searches and phylogenetic analyses indicated that it belonged to Alphapapillomavirus (Alpha-PV) species 2 and most closely resembled HPV 3. The virus fulfilled the definition of a novel type, and was named HPV 160 by the Reference Center for Papillomaviruses. The putative E7 protein of HPV 160 as well as HPV 29, 77, and 78 contained the Leu-X-Cys-X-Glu pRB-binding motif but other Alpha-PV species 2 (HPV 3, 10, 28, 94, 117, and 125) did not have this conserved motif.
DOI 10.1371/journal.pone.0079592
PMID 24282509