ナカムラ　フミオ   Fumio Nakamura   中村　史雄    所属   医学部 医学科    職種   教授・基幹分野長
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	Label-free and spectral-analysis-free detection of neuropsychiatric disease biomarkers using an ion-sensitive GaInAsP nanolaser biosensor.
掲載誌名	正式名：Biosensors & bioelectronics 略　 称：Biosens Bioelectron ISSNコード：(1873-4235)0956-5663(Linking)
掲載区分	国外
巻・号・頁	117,pp.161-167
著者・共著者	Watanabe Keisuke†, Nomoto Munetaka, Nakamura Fumio, Hachuda Shoji, Sakata Akihiro, Watanabe Takumi, Goshima Yoshio, Baba Toshihiko
発行年月	2018/10
概要	The emission intensity of GaInAsP semiconductors that show an ion sensitivity is altered by the surface charge. In this study, we propose a biosensing technique using GaInAsP photonic crystal nanolasers based on this principle. Here, simple and rapid detection of collapsin response mediator protein 2 (CRMP2) is demonstrated, which is a promising biomarker candidate for neuropsychiatric diseases existing in peripheral white blood cells. We prepared CRMP2 as a standard protein and introduced sodium dodecyl sulfate (SDS) as an anionic surfactant to enhance the net negative charge of the protein. The nanolaser was modified in advance with an anti-CRMP2 antibody and then photopumped at a constant power. The laser emission intensity was monitored during the antibody-antigen reaction. Consequently, CRMP2 was detected as a decrease in the emission intensity. We achieved a lower limit for detection that satisfies the requirement for clinical biomarker testing. Without the requirements of any kind of labels and spectral analyses, this technique allows for simple, rapid, and low-cost biomarker detection.
DOI	10.1016/j.bios.2018.05.059
PMID	29894853