ONO Masafumi
   Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine
Article types Original article
Language English
Peer review Peer reviewed
Title Abnormal lipid/lipoprotein metabolism and high plasma testosterone levels in male but not female aromatase-knockout mice.
Journal Formal name:Archives of biochemistry and biophysics
Abbreviation:Arch Biochem Biophys
ISSN code:10960384/00039861
Domestic / ForeginForegin
Volume, Issue, Page 622,pp.47-58
Author and coauthor Amano Akiko, Kondo Yoshitaka, Noda Yoshihiro, Ohta Mitsuhiro, Kawanishi Noriaki, Machida Shuichi, Mitsuhashi Kazuteru, Senmaru Takafumi, Fukui Michiaki, Takaoka Osamu, Mori Taisuke, Kitawaki Jo, Ono Masafumi, Saibara Toshiji, Obayashi Hiroshi, Ishigami Akihito
Publication date 2017/05
Summary Sex steroid hormones, such as estrogen and testosterone, are believed to play important roles in lipid metabolism. To elucidate the effects of estrogen depletion on lipid metabolism in male and female mice, we used aromatase-knockout (ArKO) mice, in which Cyp19 gene disruption prevented estrogen synthesis in vivo. These mice were divided into the following 4 groups: male and female ArKO mice and male and female wild-type (WT) mice. These mice were fed a normal-fat diet (13.6% fat) ad libitum. At 159 days after birth, the mice were tested for liver and plasma lipid content and hepatic hormone receptor- and lipid/lipoprotein metabolism-related gene expression. Interestingly, we found that hepatic steatosis was accompanied by markedly elevated plasma testosterone levels in male ArKO mice but not in female ArKO mice. Plasma lipoprotein profiles exhibited concurrent decreases in LDL- and small dense LDL-triglyceride (TG) levels in male ArKO mice. Moreover, male mice, but not female mice, exhibited marked elevations in androgen receptor (AR), sterol regulatory element-binding protein 1 (SREBP1), and CD36 expression. These results strongly suggest that Cyp19 gene disruption, which induces a sexually dimorphic response and high plasma testosterone levels in male mice, also induces hepatic steatosis.
DOI 10.1016/j.abb.2017.03.007
PMID 28341248