ONO Masafumi    Department   School of Medicine(Tokyo Women's Medical University Adachi Medical Center), School of Medicine    Position
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	17β-Estradiol is critical for the preovulatory induction of prostaglandin E(2) synthesis in mice.
Journal	Formal name：Molecular and cellular endocrinology Abbreviation：Mol Cell Endocrinol ISSN code：18728057/03037207
Domestic / Foregin	Foregin
Volume, Issue, Page	362(1-2),pp.176-82
Author and coauthor	Toda Katsumi, Ono Masafumi, Yuhki Koh-ichi, Ushikubi Fumitaka, Saibara Toshiji
Publication date	2012/10
Summary	Aromatase-deficient (ArKO) mice are totally anovulatory due to insufficient estrogen production. However, sequential administrations of high doses of 17β-estradiol (E2) and gonadotropins were found to induce ovulation in these mice. Here, we examined how the ovulatory stimulation for ArKO mice alters the expressions of genes related to prostaglandin (PG) E(2) metabolism and ovarian contents of PGE(2), as PGE(2) is one of the critical mediators of ovulatory induction. The ovulatory stimulation significantly increased mRNA expressions of prostaglandin-endoperoxide synthase 2, PGE(2) receptor type 4 and sulfotransferase family 1E, member 1, in preovulatory ArKO ovaries. In contrast, it suppressed the mRNA expression of 15-hydroxyprostaglandin dehydrogenase. Furthermore, significant elevation in the PGE(2) contents was detected in the preovulatory ovaries of ArKO mice after stimulation with E2 plus ovulatory doses of gonadotropins. Thus, these analyses demonstrate a requirement of E2 for the preovulatory enhancement of PGE(2) synthesis, leading to future success in ovulation.
DOI	10.1016/j.mce.2012.06.006
PMID	22713853