オノ　マサフミ   ONO Masafumi   小野　正文    所属   医学部 医学科（附属足立医療センター）    職種   非常勤講師
論文種別	原著
言語種別	英語
査読の有無	査読あり
表題	17β-Estradiol is critical for the preovulatory induction of prostaglandin E(2) synthesis in mice.
掲載誌名	正式名：Molecular and cellular endocrinology 略　 称：Mol Cell Endocrinol ISSNコード：18728057/03037207
掲載区分	国外
巻・号・頁	362(1-2),pp.176-82
著者・共著者	Toda Katsumi, Ono Masafumi, Yuhki Koh-ichi, Ushikubi Fumitaka, Saibara Toshiji
発行年月	2012/10
概要	Aromatase-deficient (ArKO) mice are totally anovulatory due to insufficient estrogen production. However, sequential administrations of high doses of 17β-estradiol (E2) and gonadotropins were found to induce ovulation in these mice. Here, we examined how the ovulatory stimulation for ArKO mice alters the expressions of genes related to prostaglandin (PG) E(2) metabolism and ovarian contents of PGE(2), as PGE(2) is one of the critical mediators of ovulatory induction. The ovulatory stimulation significantly increased mRNA expressions of prostaglandin-endoperoxide synthase 2, PGE(2) receptor type 4 and sulfotransferase family 1E, member 1, in preovulatory ArKO ovaries. In contrast, it suppressed the mRNA expression of 15-hydroxyprostaglandin dehydrogenase. Furthermore, significant elevation in the PGE(2) contents was detected in the preovulatory ovaries of ArKO mice after stimulation with E2 plus ovulatory doses of gonadotropins. Thus, these analyses demonstrate a requirement of E2 for the preovulatory enhancement of PGE(2) synthesis, leading to future success in ovulation.
DOI	10.1016/j.mce.2012.06.006
PMID	22713853