ムラガキ ヨシヒロ   MURAGAKI Yoshihiro
  村垣 善浩
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読なし
表題 A surgical strategy for lower grade gliomas using intraoperative molecular diagnosis.
掲載誌名 正式名:Brain tumor pathology
略  称:Brain Tumor Pathol
ISSNコード:14337398
掲載区分国外
巻・号・頁 35(3),159-167頁
著者・共著者 KORIYAMA Shunichi†, NITTA Masayuki, KOBAYASHI Tatsuya, MURAGAKI Yoshihiro, SUZUKI Akane, MARUYAMA Takashi, KOMORI Takashi, MASUI Kenta, SAITO Taiichi, YASUDA Takayuki, HOSONO Junji, OKAMOTO Saori, SHIOYAMA Takahiro, YAMATANI Hiroaki, KAWAMATA Takakazu
発行年月 2018/07
概要 Lower grade gliomas are both treated and diagnosed via surgical resection. Maximum tumor resection is currently the standard of care; however, this risks the loss of brain function. Glioma can be genetically subdivided into three different types, based on isocitrate dehydrogenase (IDH) mutation status and the presence of 1p/19q codeletion, which have radically different prognoses and responses to adjuvant therapies. Therefore, the means to identify the subtype and evaluate the surrounding tissues during surgery would be advantageous. In this study, we have developed a new surgical strategy for lower grade glioma based on the fourth edition of the World Health Organization Brain Tumor Classification, involving intraoperative molecular diagnosis. High-resolution melting analysis was used to evaluate IDH mutational status, while rapid immunohistochemistry of p53 and alpha-thalassemia/mental retardation syndrome X-linked (ATRX) was used to evaluate the 1p/19q codeletion status, allowing genetic classification during surgery. In addition, intraoperative flow cytometry was used to evaluate the surgical cavity for additional tumor lesions, allowing maximal resection while mitigating the risk of functional losses. This strategy allows the rapid intraoperative diagnosis and mapping of lower grade gliomas, and its clinical use could dramatically improve its prognosis.
DOI 10.1007/s10014-018-0324-1
PMID 29980868