ムラガキ ヨシヒロ   MURAGAKI Yoshihiro
  村垣 善浩
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 日本語
査読の有無 査読あり
表題 術中迅速フローサイトメトリーシステムの開発
−神経膠腫における評価−
掲載誌名 正式名:Cytometry Research
ISSNコード:09166920
掲載区分国内
巻・号・頁 27(2),9-15頁
著者・共著者 塩山高広†, 鈴木あかね, 野村健一, 久保寛嗣, 小林直樹, 新田雅之, 丸山隆志, 村上知之, 小森隆司, 村垣善浩
発行年月 2017/11
概要 DNA ploidy analysis is one of the two principal applications of flow cytometry, along with cell surface antigen analysis. DNA ploidy analysis is positive in numerous tumors, but no clear conclusion can be obtained in some tumors. This may be attributed in part to measuring inconsistency and interpretation bias in the analysis. As the measurement method is complex for DNA ploidy analysis of solid tumors, it is difficult to conduct the analysis as a routine clinical procedure. Hence, we have developed the system named “High DNA Content Measuring Flow Cytometer” with a tissue pretreatment function, and a freeze-dried reagent kit for cell staining, in order to perform DNA ploidy analysis easily and reproducibly in non-specialized laboratories. Biopsy specimens from brain tumors of 87 glioma patients who underwent open surgery were analyzed to evaluate the utility of our system. We were able to automatically analyze the biopsy specimens with the system within ten minutes. We compared the results of the same pretreated samples between our system and the conventional method using a common flow cytometer, confirming that the malignancy index was well correlated between them (R=0.975). These results demonstrated that our fully automated DNA ploidy analyzer is useful for rapid determination of glioma presence in surgical biopsy specimens.
DOI https://doi.org/10.18947/cytometryresearch.27.2_9