MURAGAKI Yoshihiro
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Visiting Professor
Article types Original article
Language English
Peer review Peer reviewed
Title Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells
Journal Formal name：Photodiagnosis and photodynamic therapy
Abbreviation：Photodiagnosis Photodyn Ther
ISSN code：1572-1000
Domestic / ForeginForegin
Volume, Issue, Page 18,pp.213-220
Author and coauthor OSAKI Tomohiro†, KAWASE Yuki, ISEKI Hiroshi, KISHIMOTO Shinji, IKUTA Soko, MURAGAKI Yoshihiro, AZUMA Kazuo, MURAHATA Yusuke, TSUKA Takeshi , ITO Norihiko, IMAGAWA Tomohiro, OKAMOTO Yoshiharu
Publication date 2017/06
Summary BACKGROUND: Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. METHODS: We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30mug/mL NPe6 for 24h. The cells were then irradiated with 0, 5, 15, or 30mW/cm2 of laser power, and 0, 1, 5, 10, or 20J/cm2 of laser energy. Cell viability was evaluated after 24h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10Hz, 50% duty) on Meth A cells. RESULTS: The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15mW/cm2 and a light dose of 20J/cm2 were 18.6 and 5.0mug/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. CONCLUSIONS: NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.
DOI 10.1016/j.pdpdt.2017.02.007
Document No. 28232036