ムラガキ ヨシヒロ   MURAGAKI Yoshihiro
  村垣 善浩
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells
掲載誌名 正式名:Photodiagnosis and photodynamic therapy
略  称:Photodiagnosis Photodyn Ther
ISSNコード:1572-1000
掲載区分国外
巻・号・頁 18,213-220頁
著者・共著者 OSAKI Tomohiro†, KAWASE Yuki, ISEKI Hiroshi, KISHIMOTO Shinji, IKUTA Soko, MURAGAKI Yoshihiro, AZUMA Kazuo, MURAHATA Yusuke, TSUKA Takeshi , ITO Norihiko, IMAGAWA Tomohiro, OKAMOTO Yoshiharu
発行年月 2017/06
概要 BACKGROUND: Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. METHODS: We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30mug/mL NPe6 for 24h. The cells were then irradiated with 0, 5, 15, or 30mW/cm2 of laser power, and 0, 1, 5, 10, or 20J/cm2 of laser energy. Cell viability was evaluated after 24h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10Hz, 50% duty) on Meth A cells. RESULTS: The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15mW/cm2 and a light dose of 20J/cm2 were 18.6 and 5.0mug/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. CONCLUSIONS: NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.
DOI 10.1016/j.pdpdt.2017.02.007
文献番号 28232036