MURAGAKI Yoshihiro    Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine    Position   Visiting Professor
Article types	Original article
Language	English
Peer review	Peer reviewed
Title	Development of a Small-sized Leukapheresis Column for the Treatment of Inflammatory Diseases
Journal	Formal name：東京女子医科大学雑誌 ISSN code：00409022
Volume, Issue, Page	85(3),pp.93-101
Author and coauthor	SHIMAGAKI Masaaki†, SUZUKI Takashi, ISEKI Hiroshi, MURAGAKI Yoshihiro
Publication date	2015/06
Summary	Cytapheresis methods have been extended to remove cells such as leukocytes components, and are used for autoimmune disease treatment. However, the large blood volumes in conventional columns may restrict clinical use. We developed a small cytapheresis column. We evaluated its leukocyte adsorption property and inhibitory effect on gonarthritis. The priming volume was reduced to approximately one-third that of conventional columns. Leukocytes adsorbed to a mini-column for rabbits were counted following a 1-hour circulation immediately and 24 hours after the elicitation of inflammation. The diameter of joint swelling was measured at 24 hours and 48 hours. The adsorption property was found to differ; the number of removed leukocytes in the conventional column was largerimmediately after the elicitation, but was smaller at 24 hours, compared to the developed column. Thus, the developed column has a higher adsorption rate. For the inhibitory effect on gonarthritis, swelling decreased in the developed column group before and after the 24-hour circulation, compared with the sham column. Thus, the developed and conventional columns have equal inhibitory effects. These results show that the developed column improved selectivity and removal efficiency for granulocytes and monocytes and showed performance comparable to the conventional column.
NAID	110009915565