ムラガキ　ヨシヒロ   MURAGAKI Yoshihiro   村垣　善浩    所属   医学部 医学科（東京女子医科大学病院）    職種   客員教授
論文種別	原著
言語種別	日本語
査読の有無	査読あり
表題	Expression of trk receptors in the developing and adult human central and peripheral nervous system
掲載誌名	正式名：J Comp Neurol ISSNコード：0021-9967
巻・号・頁	356(3),387-397頁
著者・共著者	MURAGAKI Yoshihiro†, TIMOTHY Nigel, LEIGHT Susan, HEMPSTEAD Barbara L, CHAO Moses V., TROJANOWSKI John Q.,LEE Virginia M.-Y.
担当区分	筆頭著者
発行年月	1995/06
概要	A family of tyrosine receptor kinases known collectively as trk receptors plays an essential role in signal transduction mediated by nerve growth factor and related neurotrophins. To localize the major trk receptors (trkA, B and C) in the developing and adult central (CNS) and peripheral (PNS) nervous system, we generated monoclonal antibodies (MAbs) to extracellular (MAbs E7, E13, E16, E21, E29) and intracellular (MAb I2) domains of human trkA fused to glutathione S-transferase. Several MAbs (E7, E13, E16) recognized glycosylated trkA (gp140trk and gp110trk) in Western blots, one MAb (E7) recognized non-glycosylated (p80trk) and glycosylated trkA in immunoprecipitation assays, and two MAbs (E13, E29) detected trkA on the cell surface of NIH3T3 cells transfected with a trkA cDNA. Although generated to trkA fusion proteins, this panel of MAbs also recognized trkB and trkC in flow cytometric studies of NIH3T3 cells transfected with trkB or trkC cDNAs.
DOI	10.1002/cne.903560306
文献番号	7642800