ムラガキ ヨシヒロ   MURAGAKI Yoshihiro
  村垣 善浩
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 日本語
査読の有無 査読あり
表題 Expression of trk receptors in the developing and adult human central and peripheral nervous system
掲載誌名 正式名:J Comp Neurol
ISSNコード:0021-9967
巻・号・頁 356(3),387-97頁
著者・共著者 MURAGAKIYoshihiro, TIMOTHYNigel, LEIGHTSusan, HEMPSTEADBarbara L, CHAOMoses V., TROJANOWSKIJohn Q., LEEVirginia M.‐Y.
発行年月 1995/06
概要 A family of tyrosine receptor kinases known collectively as trk receptors plays an essential role in signal transduction mediated by nerve growth factor and related neurotrophins. To localize the major trk receptors (trkA, B and C) in the developing and adult central (CNS) and peripheral (PNS) nervous system, we generated monoclonal antibodies (MAbs) to extracellular (MAbs E7, E13, E16, E21, E29) and intracellular (MAb I2) domains of human trkA fused to glutathione S-transferase. Several MAbs (E7, E13, E16) recognized glycosylated trkA (gp140trk and gp110trk) in Western blots, one MAb (E7) recognized non-glycosylated (p80trk) and glycosylated trkA in immunoprecipitation assays, and two MAbs (E13, E29) detected trkA on the cell surface of NIH3T3 cells transfected with a trkA cDNA. Although generated to trkA fusion proteins, this panel of MAbs also recognized trkB and trkC in flow cytometric studies of NIH3T3 cells transfected with trkB or trkC cDNAs.
DOI 10.1002/cne.903560306
文献番号 7642800