ムラガキ ヨシヒロ   MURAGAKI Yoshihiro
  村垣 善浩
   所属   研究施設 研究施設
   職種   教授
論文種別 原著
言語種別 英語
査読の有無 査読あり
表題 Endoscopic cell sheet transplantation device developed by using a 3-dimensional printer and its feasibility evaluation in a porcine model
掲載誌名 正式名:Gastrointestinal endoscopy
略  称:Gastrointest Endosc
ISSNコード:10976779 (Electronic)00165107 (Linking)
巻・号・頁 82(1),147-152頁
著者・共著者 MAEDA Masanori†, KANAI Nobuo*, KOBAYASHI Shinichiro, HOSOI Takahiro, TAKAGI Ryo, OHKI Takashi, MURAGAKI Yoshihiro, YAMATO Masayuki*, EGUCHI Susumu, FUNAI Fumio, OKANO Teruo
発行年月 2015/07
概要 BACKGROUND: To prevent severe esophageal stenosis after aggressive endoscopic submucosal dissection (ESD), our group previously reported an efficient treatment using cell sheets that had been fabricated from patient cells. However, this transplantation procedure had not been easy for every endoscopist and needed to be improved to derive the full effect of epithelial cell sheets. OBJECTIVE: To develop an endoscopic device that enables easy and effective cell sheet transplantation and to evaluate its performance and clinical feasibility. DESIGN: Animal study. SETTING: Animal experimentation laboratory. INTERVENTION: Three pigs underwent circumferential esophageal ESD while under general anesthesia. A total of 12 cell sheets were endoscopically transplanted to the ESD site; 6 cell sheets were transplanted by using an endoscopic device that we developed, and 6 cell sheets were transplanted by using the conventional method. MAIN OUTCOME MEASUREMENTS: Procedure time, transplanted area on the ESD site, transplantation success rate, and monitoring of adverse events or incidents. RESULTS: The device allowed successful transplantation of all cell sheets with a shorter procedure time than with the conventional method (4.8 +/- 0.8 minutes vs 13.3 +/- 5.7 minutes, respectively) (P = .005) and onto a larger area (111.3 +/- 56.3 mm2 vs 41.8 +/- 4.2 mm2, respectively) (P = .023) with a higher success rate (100% vs 83%, respectively). No adverse incidents were monitored in each method. LIMITATIONS: Animal study, small sample. CONCLUSION: A newly designed endoscopic cell sheet transplantation device would be useful.
DOI 10.1016/j.gie.2015.01.062
文献番号 25892058