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Nanke Yuki
Department School of Nursing, School of Nursing Position Professor |
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| Article types | Original article |
| Language | English |
| Peer review | Peer reviewed |
| Title | RANK Expression and Osteoclastogenesis in Human Monocytes in Peripheral Blood from Rheumatoid Arthritis Patients. |
| Journal | Formal name：BioMed research international Abbreviation：Biomed Res Int ISSN code：(2314-6141) |
| Domestic / Foregin | Foregin |
| Volume, Issue, Page | 2016,pp.4874195 |
| Author and coauthor | Nanke Yuki, Kobashigawa Tsuyoshi, Yago Toru, Kawamoto Manabu, Yamanaka Hisashi, Kotake Shigeru |
| Publication date | 2016 |
| Summary | Rheumatoid arthritis (RA) appears as inflammation of synovial tissue and joint destruction. Receptor activator of NF-κB (RANK) is a member of the TNF receptor superfamily and a receptor for the RANK ligand (RANKL). In this study, we examined the expression of RANKhigh and CCR6 on CD14+ monocytes from patients with RA and healthy volunteers. Peripheral blood samples were obtained from both the RA patients and the healthy volunteers. Osteoclastogenesis from monocytes was induced by RANKL and M-CSF in vitro. To study the expression of RANKhigh and CCR6 on CD14+ monocytes, two-color flow cytometry was performed. Levels of expression of RANK on monocytes were significantly correlated with the level of osteoclastogenesis in the healthy volunteers. The expression of RANKhigh on CD14+ monocyte in RA patients without treatment was elevated and that in those receiving treatment was decreased. In addition, the high-level expression of RANK on CD14+ monocytes was correlated with the high-level expression of CCR6 in healthy volunteers. Monocytes expressing both RANK and CCR6 differentiate into osteoclasts. The expression of CD14+RANKhigh in untreated RA patients was elevated. RANK and CCR6 expressed on monocytes may be novel targets for the regulation of bone resorption in RA and osteoporosis. |
| DOI | 10.1155/2016/4874195 |
| PMID | 27822475 |