KEN OKAZAKI
   Department   School of Medicine(Tokyo Women's Medical University Hospital), School of Medicine
   Position   Professor and Division head
Article types Original article
Language English
Peer review Peer reviewed
Title Characterisation of osteophytes as an autologous bone graft source: An experimental study in vivo and in vitro.
Journal Formal name：Bone & joint research
Abbreviation：Bone Joint Res
ISSN code：20463758/20463758
Domestic / ForeginForegin
Volume, Issue, Page 6(2),pp.73-81
Author and coauthor Ishihara K, †, Okazaki K*, Akiyama T, Akasaki Y, Nakashima Y
Authorship 2nd author,Corresponding author
Publication date 2017/02
Summary OBJECTIVES:Osteophytes are products of active endochondral and intramembranous ossification, and therefore could theoretically provide significant efficacy as bone grafts. In this study, we compared the bone mineralisation effectiveness of osteophytes and cancellous bone, including their effects on secretion of growth factors and anabolic effects on osteoblasts.METHODS:Osteophytes and cancellous bone obtained from human patients were transplanted onto the calvaria of severe combined immunodeficient mice, with Calcein administered intra-peritoneally for fluorescent labelling of bone mineralisation. Conditioned media were prepared using osteophytes and cancellous bone, and growth factor concentration and effects of each graft on proliferation, differentiation and migration of osteoblastic cells were assessed using enzyme-linked immunosorbent assays, MTS ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)) assays, quantitative real-time polymerase chain reaction, and migration assays.RESULTS:After six weeks, the area of mineralisation was significantly higher for the transplanted osteophytes than for the cancellous bone (43803 μm2, sd 14660 versus 9421 μm2, sd 5032, p = 0.0184, one-way analysis of variance).
DOI 10.1302/2046-3758.62.BJR-2016-0199.R1
PMID 28148490